| 不同抗逆诱导剂对水稻细胞PBZ 1 基因表达的影响 |
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| 引用本文： | 丛林晔,丁秀英,王毓,何永明,陈淑君,张军.不同抗逆诱导剂对水稻细胞PBZ 1 基因表达的影响[J].农药学学报,2005,7(3):254-258. |
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| 作者姓名： | 丛林晔 丁秀英 王毓 何永明 陈淑君 张军 |
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| 作者单位： | 1.中国农业大学 生物学院,北京 100094 |
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| 摘 要： | 以水稻品种中花8号的悬浮细胞为试材,分别研究了真菌激发子和盐激发子对用稻瘟灵(IPT)和脱落酸(ABA)预处理24 h的水稻悬浮细胞中PBZ 1 基因表达的影响。结果发现:单独用IPT、ABA处理可以诱导细胞中PBZ 1 基因表达;只用真菌激发子和盐激发子处理的细胞中也可以检测到PBZ 1 基因的表达,但两种激发子对PBZ 1 基因表达的影响不同,在真菌激发子处理后的0~3 h之间PBZ 1 基因表达逐渐增强,而在盐激发子处理后的0~3 h之间则呈现逐渐减弱的趋势,到第3 h就检测不到了;用IPT预处理24 h后,再分别用真菌激发子和盐激发子诱导,水稻细胞中PBZ 1 基因表达强度都显著高于单独用IPT处理的细胞,而且在处理后的第0.5~3 h期间一直保持较强的表达;ABA＋盐激发子处理使细胞中PBZ 1 基因表达推迟且短暂,只在处理后第1~2 h之间可检测到,ABA＋真菌激发子处理使细胞中PBZ 1 基因在0.5~3 h期间一直保持较强的表达,明显强于单独用ABA处理的细胞。可见,IPT和ABA都可以诱导抗病基因PBZ 1 的表达,PBZ 1 基因也可由盐激发子诱导表达,说明PBZ 1 基因对除病害以外的非生物逆境也有积极的作用。
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| 关 键 词： | 抗逆诱导剂 稻瘟灵 脱落酸 PBZ 1 基因 |
| 文章编号： | 1008-7303（2005）03-0254-05 |
| 收稿时间： | 2004-11-10 |
| 修稿时间： | 2005-05-10 |
Effect on Expression of PBZ 1 in Rice Suspension Cell by Different Stress-resistance Inducers |
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| CONG Lin-ye,DING Xiu-ying,WANG Yu,HE Yong-ming,CHEN Shu-jun and ZHANG Jun.Effect on Expression of PBZ 1 in Rice Suspension Cell by Different Stress-resistance Inducers[J].Chinese Journal of Pesticide Science,2005,7(3):254-258. |
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| Authors: | CONG Lin-ye DING Xiu-ying WANG Yu HE Yong-ming CHEN Shu-jun and ZHANG Jun |
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| Institution: | 1.College of Biology Science, China Agricultural University, Beijing 100094,China |
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| Abstract: | The effect on expression of PBZ1 in rice suspension cell (Zhonghua 8)induced by the elicitors of fungal and salt pretreated with isoprothiolane (IPT) and abscisic acid (ABA) for 24 h were studied. The results showed that IPT or ABA or the two elicitors treatment could separately (induce) the expression of PBZ1 in the suspension cell and without the expression of PBZ1 in the (control) cells was observed treated with only the fungal and salt elicitors.The expression of PBZ1 (enhanced) gradually from zero to three hours after treated with fungal elicitor and weakened gradually to zero within the same interval treated by salt elicitor. The expression intensity of PBZ1 in rice cell treated only with IPT was obviously higher than that of induced by elicitor of fungal or salt pretreated by IPT for 24 h, and a continuance of high expression of PBZ1 was observed from 0.5 to (3 h) after (treatment) as well as by fungal elicitor after ABA treatment. It is obvious that IPT, ABA and fungal could induce the expression of PBZ1. The PBZ1 gene had active effects on the abiotic stresses with the exception of diseases. |
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| Keywords: | stress-resistance inducer isoprothiolane (IPT) abscisic acid (ABA) PBZ1 |
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