| 中国野生毛葡萄芪合酶基因VqSTS11和VqSTS23调控抗白粉病的研究 |
| |
| 引用本文： | 赵凯茜,丁 茜,王跃进.中国野生毛葡萄芪合酶基因VqSTS11和VqSTS23调控抗白粉病的研究[J].园艺学报,2020,47(7):1264-1276. |
| |
| 作者姓名： | 赵凯茜 丁 茜 王跃进 |
| |
| 作者单位： | 西北农林科技大学园艺学院，旱区作物逆境生物学国家重点实验室，农业部西北地区园艺作物生物与种质创制重点实验室，陕西杨凌 712100 |
| |
| 基金项目： | 国家自然科学基金项目（31872055） |
| |
| 摘 要： | 利用同源克隆法得到抗白粉病的中国野生毛葡萄‘丹凤–2’芪合酶基因VqSTS11和VqSTS23的开放阅读框，并构建过表达载体；通过器官发生途径诱导不抗白粉病的欧洲葡萄‘无核白’分生愈伤组织并采用农杆菌介导法对其进行转化；经过PCR检测和Western blot鉴定，获得了5株VqSTS11超量表达植株和3株VqSTS23超量表达植株；人工接种白粉菌发现，转基因植株中白粉菌菌丝生长速度慢，孢子萌发受到一定的抑制，并且转基因植株中芪合酶基因相对表达量升高，抗病相关基因表达上调，芪类物质含量积累增多。本研究结果进一步证明中国野生毛葡萄‘丹凤–2’芪合酶基因VqSTS11和VqSTS23对白粉菌具有抗性，‘丹凤–2’可以为改良欧洲葡萄品种抗病性提供抗病基因，作为抗病育种的资源。
|
| 关 键 词： | 毛葡萄 欧洲葡萄 芪合酶 白藜芦醇 白粉病 抗病性 转基因 |
Research on Control of Disease Resistance by Stilbene Synthase Genes Derived from Chinese wild Vitis quinquangularis |
| |
| ZHAO Kaixi,DING Xi,WANG Yuejin.Research on Control of Disease Resistance by Stilbene Synthase Genes Derived from Chinese wild Vitis quinquangularis[J].Acta Horticulturae Sinica,2020,47(7):1264-1276. |
| |
| Authors: | ZHAO Kaixi DING Xi WANG Yuejin |
| |
| Institution: | College of Horticulture，Northwest A & F University，State Key Laboratory of Crop Stress Biology in Arid Areas，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture，Yangling，Shaanxi 712100，China |
| |
| Abstract: | Two complete open reading frames of VqSTS11 and VqSTS23，were respectively isolated from Vitis quinquangularis‘Danfeng-2’using homologous cloning technique and further for overexpression vector construction. Meristematic bulk（MB）of cultivated Vitis vinifera‘Thompson Seedless’，were induced through organogenesis and further for Agrobacterium-mediated transformation to obtain transgenic plants. After PCR detection and Western blot identification，5 transgenic lines of VqSTS11 and 3 transgenic lines of VqSTS23 were verified，respectively. Transgenic plants and non-transgenic plants were artificially inoculated with powdery mildew. Microscopic observation demonstrated that the hyphae growth in transgenic lines were slower than those in non-transgenic plants and spore germination was inhibited in transgenic plants. The disease resistance of transgenic plants was further analyzed by qRT-PCR and HPLC. The results displayed that not only the expression of STSs in transgenic plants increased，and the expression of disease resistance-related genes was up-regulated，but also stilbene compounds in the transgenic plants were accumulated. The expression product of‘Danfeng-2’VqSTS11 and VqSTS23 could tolerate powdery mildew. Therefore，‘Danfeng-2’could be used to improve the disease tolerance and provide disease- tolerant candidate genes for European grape cultivars，which could be served as a breeding resources for disease-tolerant breeding. |
| |
| Keywords: | Vitis quinquangularis Vitis vinifera stilbene synthase resveratrol powdery mildew disease resistance transgenic |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
