| 萱草叶斑病的病原鉴定及其生物学特性 |
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| 引用本文： | 高 晋,曾桂萍,宋莉莎,赵 致,李 忠.萱草叶斑病的病原鉴定及其生物学特性[J].园艺学报,2020,47(1):169-178. |
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| 作者姓名： | 高 晋 曾桂萍 宋莉莎 赵 致 李 忠 |
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| 作者单位： | 1贵州大学农学院，贵阳 550025；2贵州省药用植物繁育与种植重点实验室，贵阳 550025 |
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| 基金项目： | 贵州省人才基地建设项目（黔人领发[2013]15号）；科技部科技惠民计划项目（2013GS520101-08） |
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| 摘 要： | 为明确在贵州发现的萱草（Hemerocallis fulva）叶斑病病原菌及其生物学特性，采用组织分离法和离体接种法分别对其病原菌进行分离和致病性测定，利用形态学及ITS基因序列分析对病原菌进行鉴定并对其生物学特性进行研究。鉴定结果表明：分离得到的萱草叶斑病病原菌菌株（编号为XCS369）的菌丝为灰白色、绒毛状，菌落中心隆起呈灰褐色，背面橄榄绿色，培养15 d表面可产生白色孢子粉；分生孢子光滑，无色，椭圆至长椭圆形。在以ITS基因序列构建的系统发育树中，XCS369与古巴炭角菌（Xylaria cubensis）聚于一支，且支持率达99%，结合形态特征与系统发育分析将其鉴定为古巴炭角菌。该菌菌丝最适生长温度为25 ~ 28 ℃，最适pH 7，在马铃薯葡萄糖培养基上生长最快，对碳源麦芽糖、氮源酵母浸粉的利用最高，菌丝生长对光照不敏感。
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| 关 键 词： | 萱草 叶斑病 病原鉴定 古巴炭角菌 生物学特性 |
Identification and Biological Characteristics of Pathogen Causing Leaf Spot Disease of Hemerocallis fulva |
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| GAO Jin,ZENG Guiping,SONG Lisha,ZHAO Zhi,LI Zhong.Identification and Biological Characteristics of Pathogen Causing Leaf Spot Disease of Hemerocallis fulva[J].Acta Horticulturae Sinica,2020,47(1):169-178. |
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| Authors: | GAO Jin ZENG Guiping SONG Lisha ZHAO Zhi LI Zhong |
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| Institution: | 1.Agriculture College，Guizhou University，Guiyang 550025，China；2Guizhou Key Laboratory of Medicine Plants Breeding and Cultivation，Guiyang 550025，China |
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| Abstract: | To clarify the pathogen causing leaf spots of Hemerocallis fulva in Guizhou Province and its biological characteristic，the pathogen was obtained by using the method of tissue isolation. Its pathogenicity was tested in vitro inoculated leaves，and the pathogen was classified and identified with morphology and ITS gene sequence analyse，the biological characteristic of pathogen were also studied. The results showed that the strain isolated from leaf brown spots of Hemerocallis fulva was numbered as XCS369，its main diagnostic feature were as followed：the mycelia was grayish white and villous，colony center ridge is taupe，back olive green，and the back of colonies were olive green. After 15 days of cultivation，it could produce white sporangium powder. Its smooth and colorless conidia appeared elliptical or oblong. The phylogenetic tree based on ITS gene sequence showed that XCS369 strain was clustered together with Xylaria cubensis，supported by 99%. Together with the morphology characteristics and phylogenetic analysis，it was identified as one member of Xylaria cubensis. The research of biological characteristics showed that the mycelia of XCS369 strain grew faster on potato dextrose medium of pH 7 and had the highest utilization of carbon source maltose and niteogen source yeast extract powder at 25–28 ℃，and it was insensitive to light. |
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| Keywords: | Hemerocallis fulva leaf spot disease pathogens identification Xylaria cubensi biological characteristics |
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