| 番茄/茄子嫁接对其根际土壤生物学性状及细菌群落结构的影响 |
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| 引用本文： | 庞师婵,郭,霜,任奎瑜,王帅帅,杨尚东.番茄/茄子嫁接对其根际土壤生物学性状及细菌群落结构的影响[J].园艺学报,2020,47(2):253-263. |
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| 作者姓名： | 庞师婵 郭 霜 任奎瑜 王帅帅 杨尚东 |
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| 作者单位： | 1 广西大学农学院，植物科学国家级实验教学示范中心，南宁 530004；2 广西农业科学院，广西甘蔗遗传改良重点实验室，南宁 530007 |
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| 基金项目： | 国家自然科学基金项目（31860547）；广西科技重大专项资金项目（AA17204041）；亚热带农业生物资源保护与利用国家重点实验室开放课题（OSKL201506） |
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| 摘 要： | 以茄子作为砧木，番茄为接穗进行嫁接，并以番茄自根植株和茄子自根植株作为对照，基于传统及现代高通量测序技术分析根际土壤的生物学性状及细菌群落结构特征，旨在揭示嫁接植株抗性增强的机制。结果表明：番茄/茄子嫁接植株根际土壤中可培养细菌、放线菌数量，微生物生物量、酶活性和细菌丰富度、多样性指数均显著高于茄子和番茄自根植株；另一方面，norank_c_Acidobacteria、硝化螺菌属（Nitrospira）、芽孢杆菌属（Bacillus）、norank_f_Gemmatimonadaceae、norank_o_Gaiellales、norank_f_Xanthobacteraceae、norank_o_SC-I-84、norank_f_Anaerolineaceae、norank_f_Nitrosomonadaceae、鞘脂单胞菌属（Sphingomonas）细菌是嫁接植株与茄子、番茄自根植株根际土壤中的共有优势细菌属；g_unclassified_o_Ignavibacteriales 、 醋酸杆菌属（ g_Acetobacter ）、g_norank_f_Longimicrobiaceae 、g_unclassified_f_Verrucomicrobiaceae、g_norank_p_FBP、g_norank_p_Aminicenantes 属细菌是嫁接植株根际土壤的特有优势细菌属。与自根植株相比，番茄/茄子嫁接植株根际土壤存在更高可培养微生物数量，微生物生物量和相关酶活性，具有更高的土壤养分有效性，同时拥有不同占比的共有优势细菌门属，尤其拥有更为丰富的微生物可操作分类单元（operational taxonomic units，OTU）和特有 OTU 数量，这是嫁接植株抗性增强的主要原因。
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| 关 键 词： | 番茄 嫁接 土壤生物学性状 细菌群落结构 高通量测序 |
Impact of Grafting on Soil Microbial Properties and Bacterial Community Structure in Tomato Rhizosphere |
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| PANG Shichan,GUO Shuang,REN Kuiyu,WANG Shuaishuai,YANG Shangdong..Impact of Grafting on Soil Microbial Properties and Bacterial Community Structure in Tomato Rhizosphere[J].Acta Horticulturae Sinica,2020,47(2):253-263. |
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| Authors: | PANG Shichan GUO Shuang REN Kuiyu WANG Shuaishuai YANG Shangdong |
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| Institution: | 1.National Experimental Teaching Demonstration Center of Plant Science，Agricultural College，Guangxi University，Nanning 530004，China；2Guangxi Key Laboratory of Sugarcane Genetic Improvement，Guangxi Academy of Agricultural Sciences，Nanning 530007，China |
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| Abstract: | Grafting was found effective in enhancing plant disease resistance. Rhizosphere microbial communities play a crucial role in plant resistance to soil-borne diseases. Eggplants and tomato were used as rootstocks and scions，respectively，to investigate the impact of grafting on soil biochemical properties and bacterial community composition in tomato rhizosphere. We found that grafting significantly increased cultivable bacteria，actinomycetes，microbial biomass-C，N and P，and enzyme activities in tomato rhizosphere. Higher bacterial richness，evenness and diversity indices were also observed in the rhizosphere of grafted plants compare to self-rooted plants. High-throughput sequencing revealed that norank_c_Acidobacteria，Nitrospira，Bacillus，norank_f_Gemmatimonadaceae，norank_o_Gaiellales，norank_f_Xanthobacteraceae，norank_o_SC-I-84，norank_f_Anaerolineaceae，norank_f_Nitrosomonadaceae and Sphingomonas were dominant bacterial genera，which were found common in rhizosphere of grafted and self-rooted plants. The g_unclassified_o_Ignavibacteriales，g_Acetobacter，g_norank_f_ Longimicrobiaceae，g_unclassified_f_Verrucomicrobiaceae，g_norank_p_FBP，g_norank_p，and Aminicenantes were specific dominant bacterial genera found only in the rhizosphere of grafted plants. Our results indicated that tomato grafted on eggplant promoted soil nutrition availability by increasing culturable microorganisms，microbial biomass and related enzyme，activities in tomato rhizosphere. At the same time，it has different dominant proportions of the common bacterial genus,especially greater number of bacterial total OTU and endemic OTU，which is the main reason for the increased resistance of grafted plants. |
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| Keywords: | tomato grafting soil biological properties bacterial community structure high throughput sequencing |
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