| 一株红霉素降解菌红圆酵母的选育及其降解特性 |
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| 引用本文： | 王敏奇,许晓玲,李卫芬,许梓荣,马玉龙.一株红霉素降解菌红圆酵母的选育及其降解特性[J].农业生物技术学报,2009,17(2):341-346. |
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| 作者姓名： | 王敏奇 许晓玲 李卫芬 许梓荣 马玉龙 |
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| 作者单位： | 1. 浙江大学动物科学学院,杭州,310029
2. 宁夏大学化学化工学院,银川,750000 |
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| 基金项目： | 教育部新世纪优秀人才支持计划 |
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| 摘 要： | 选取长期堆放红霉素药渣的土壤，经驯化富集后筛选到1株能高效降解红霉素的菌株，根据表型特征、生理生化特性及18S rDNA序列同源性分析，鉴定为红酵母属的粘性红圆酵母（Rhodotorula mucilaginosa）。该菌的18S rDNA序列片段长度为1 562 bp，在GenBank登录号为EU294522。该菌可以利用红霉素作为碳源生长，适用碳源与氮源分别为蔗糖和NH4Cl。该菌降解红霉素的最适温度为30 ℃，最适pH 5.0～5.5，最适接菌量6%，降解率与通气量成正相关。在最适反应条件下培养48 h, 该菌株对红霉素的降解率可达100%。
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| 关 键 词： | 红霉素降解菌 选育 降解特性 |
| 收稿时间： | 2008-5-5 |
| 修稿时间： | 2008-5-31 |
Screening of An Erythromycin-degrading Strain Rhodotorula and Its Degradation Characteristics |
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| WANG Min-qi,XU Xiao-ling,LI Wei-fen,XU Zi-rong,MA Yu-long.Screening of An Erythromycin-degrading Strain Rhodotorula and Its Degradation Characteristics[J].Journal of Agricultural Biotechnology,2009,17(2):341-346. |
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| Authors: | WANG Min-qi XU Xiao-ling LI Wei-fen XU Zi-rong MA Yu-long |
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| Abstract: | An erythromycin-degrading strain was isolated from soil contaminated by erythromycin. Based on analysis of its morphology, 18S rDNA, physiological and biochemical characteristics, the strain was identified to be Rhodotorula mucilaginosa. Its sequence of 18S rDNA was 1 562 bp and the accession number was EU294522 in GenBank. The strain could grow using erythromycin as the carbon source, optimal carbon and nitrogen sources were sucrose and NH4Cl, respectively. The optimal temperature, pH and inoculation for erythromycin degradation were 30 ℃, 5~5.5, and 6% , respectively, and he degradation rate was positively related to aeration, and could be 100% after the strain was cultured for 48 h. |
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