| CAV基因T程亚单位苗与IBDV二联灭活疫苗的研究 |
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| 引用本文： | 张知良,黄建芳,赵宝华,胡清海,何家禄,张志芳,肖庆利.CAV基因T程亚单位苗与IBDV二联灭活疫苗的研究[J].动物医学进展,2002,23(3):57-59. |
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| 作者姓名： | 张知良 黄建芳 赵宝华 胡清海 何家禄 张志芳 肖庆利 |
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| 作者单位： | 1. 江苏省家禽科学研究所,江苏扬州,225003
2. 中国农业科学院蚕桑研究所,江苏镇江,212000 |
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| 基金项目： | 江苏省"九五"农业科技计划项目(BE96457) |
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| 摘 要： | IBDV为自行分离的VVIBDV COB－C1组织毒,毒价为CELD5010^5.0/0.2mL,CAV为VP1和VP2基因克隆进家蚕杆状病毒转基因载体质粒中,后经重组,筛选后获得的重组VP1和VP2基因产物,IBDV经甲醛灭活后与CAV按适当比例混合。1：4与白油佐剂研制成油包水型乳化剂二联疫苗,经安全试验、免疫保护试验证明该二联灭活疫苗安全有效,免疫种鸡群后可使其后代产生IBDV和CAV抗体,雏鸡得到较好的保护。
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| 关 键 词： | 鸡传染性贫血病病毒 传染性法氏囊病毒 CAV 基因工程亚单位苗 IBDV二联灭活疫苗 |
| 文章编号： | 1007-5038(2002)03-0057-03 |
| 修稿时间： | 2001年10月22 |
Study of the Bigeminal Vaccine Included CAV Subunit Vaccine and Inactivated IBDV |
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| ZHANG Zhi liang ,HUANG Jian fang ,ZHAO Bao hua,HU Qing hai ,HE Jia lu ,ZHANG Zhi fang ,XIAO Qing li.Study of the Bigeminal Vaccine Included CAV Subunit Vaccine and Inactivated IBDV[J].Progress In Veterinary Medicine,2002,23(3):57-59. |
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| Authors: | ZHANG Zhi liang HUANG Jian fang ZHAO Bao hua HU Qing hai HE Jia lu ZHANG Zhi fang XIAO Qing li |
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| Institution: | ZHANG Zhi liang 1,HUANG Jian fang 1,ZHAO Bao hua,HU Qing hai 1,HE Jia lu 2,ZHANG Zhi fang 2,XIAO Qing li 2 |
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| Abstract: | A strain of VVIBDV COB C1 was isolated from the tissue infected by IBD. The ELD 50 of the isolate was 10 5.6 /0.2 mL. The genes of VP1 and VP2 of CAV were cloned into baciulliform virus vectors. The IBDV were mixed up with CAV according to appropriate proportion after the active was destroyed by formaldehyde. The bigeminal vaccine were made according general methods. The protect test indicated that the vaccine were safty and useful. After the breeded chicken were immunized, the antibody against IBDV and CAV can be detected from the descendant. |
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| Keywords: | CAV IBDV gene engineering subunit vaccine |
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