| 木薯炭疽病病原鉴定及其生物学特性研究（摘要）（英文） |
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| 引用本文： | 蔡吉苗,李超萍,时涛,林春花,黄贵修.木薯炭疽病病原鉴定及其生物学特性研究（摘要）（英文）[J].农业科学与技术,2010,11(3):129-134. |
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| 作者姓名： | 蔡吉苗 李超萍 时涛 林春花 黄贵修 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南儋州571737 |
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| 基金项目： | 国家现代产业体系木薯病虫害普查及其重要外来有害生物预警研究项目（nycytx-17-37）;农业部公益行业木薯技术体系科学家岗位研究项目（nyhyzx07-013-5）. |
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| 摘 要： | 目的]分离鉴定木薯炭疽病菌,并进行生物学特性研究。方法]从我国海南木薯发病叶片上分离到2株木薯炭疽病菌CCGHN01和CCGHN03,分离物的致病性采用针刺法进行确认,通过分生孢子形态观察和ITS序列分析进行鉴定。采用CTAB法提取2个木薯炭疽病菌菌株的基因组DNA,用通用引物ITS1和ITS4进行PCR扩增。扩增产物克隆后各挑3个阳性克隆测序后进行比对以确认其序列,序列在http：//www.ncbi.nlm.nih.gov/上进行比较分析。研究2个木薯炭疽菌株的生物学特性,各影响因子包括：培养基种类（马铃薯琼脂葡萄糖、马铃薯琼脂蔗糖、玉米粉、燕麦、麦芽、胡萝卜培养基）,菌落生长温度（5、10、15、20、24、26、28、30、33、37、40℃）、pH值（4.0、5.0、6.0、7.0、8.0、9.0、10.0、11.0、12.0）、光照（完全黑暗、完全光照和光暗交替）,分生孢子萌发温度（5、10、15、20、24、26、28、30、33、37和40℃）、致死温度（40、45、50、55、60和65℃）。结果]分生孢子形态观察和ITS序列分析表明2株病原菌为胶孢炭疽菌。生物学特性研究表明,2个菌株生长最适培养基为马铃薯琼脂蔗糖培养基,最适温度分别是26和30℃,最适pH值为8.0,最适光照条件分别是光暗交替和完全黑暗。2个菌株孢子萌发最适温度分别是28和30℃,致死温度为55℃ 10 min。结论]该研究为进一步防治木薯炭疽病提供了基础。
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| 关 键 词： | 木薯 炭疽病 生物学特性 |
Study on Pathogeny Identification and Biological Characteristics of Anthracnose in Cassava |
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| CAI Ji-miao,LI Chao-ping,SHI Tao,LIN Chun-hua,HUANG Gui-xiu.Study on Pathogeny Identification and Biological Characteristics of Anthracnose in Cassava[J].Agricultural Science ＆ Technology,2010,11(3):129-134. |
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| Authors: | CAI Ji-miao LI Chao-ping SHI Tao LIN Chun-hua HUANG Gui-xiu |
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| Institution: | Key Laboratory for Baleful Biology Detection and Monitoring of Tropical Agriculture of Hainan Province,Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou 571737 |
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| Abstract: | Objective] The research aimed to isolate and identify the anthracnose pathogen of Cassava and study its biological charcteristics.Method] Two isolates of anthracnose （CCGHN01 and CCGHN03） in Cassava were isolated from the diseased leaves collected from Hainan of China.They were identified through the morphological observation of conidia and ITS sequence analysis.And the morphological characteristics were studied.Result] The morphological observation of conidia and ITS sequence analysis indicated that the two isolates were Colletotrichum gloeosporioides.The morphological characteristics study results showed that optimum medium for the growth of two strains was PSA,the optimum temperatures were 26 ℃ and 30 ℃ respectively,the optimum pH was 8.0,the optimum light conditions were alternative light and dark and complete darkness respectively.For the conidia germination of two strains,the optimum temperatures were 28 ℃ and 30 ℃ respectively and the lethal temperature was 55 ℃ remaining 10 min.Conclusion] The research laid the foundation for further control of anthracnose in Cassava. |
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| Keywords: | Cassava Anthracnose Biological characteristics |
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