| 大蒜头提取物对丁酰胆碱酯酶抑制作用的测定分析 |
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| 引用本文： | 谢娜娜,陈福生,路磊.大蒜头提取物对丁酰胆碱酯酶抑制作用的测定分析[J].湖北农业科学,2009,48(1). |
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| 作者姓名： | 谢娜娜 陈福生 路磊 |
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| 作者单位： | 华中农业大学食品科技学院,武汉,430070 |
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| 基金项目： | 武汉市产业化专项基金 |
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| 摘 要： | 试验采用蒸馏水提取得到的大蒜头中干扰酶抑制剂检测有机磷与氨基甲酸酯农药残留的提取物,以2,6-二氯靛酚作为显色剂,分析了提取物对丁酰胆碱酯酶活性的抑制类型.结果表明,大蒜头提取物对丁酰胆碱酯酶的抑制为可逆型抑制.
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| 关 键 词： | 大蒜头 2 6-二氯靛酚法 丁酰胆碱酯酶 |
Study on the Inhibition Mechanism of Garlic Extracts to Cholinesterase |
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| XIE Na-na,CHEN Fu-sheng,Lu Lei.Study on the Inhibition Mechanism of Garlic Extracts to Cholinesterase[J].Hubei Agricultural Sciences,2009,48(1). |
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| Authors: | XIE Na-na CHEN Fu-sheng Lu Lei |
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| Institution: | College of Food Science and Technology;Huazhong Agricultural University;Wuhan 430070;China |
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| Abstract: | The substances disturbing enzyme inhibition method of detecting organophosphate and carbamate pesticide residues were extracted with water from garlic.The inhibition type of the extraction on butanoyl-cholinesterase was analyzed with the color developing agent of 2,6-dicholoro-indophenoltitration.The results revealed that the inhibition of garlic extraction on butanoylcholinesterase was reversible,which laid a foundation for further researches on the cholinesterase inhibition methods of detecting orgabiogis... |
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| Keywords: | garlic 2 6-dichloro-indophenoltitration method butyrylcholinesterase |
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