| 湖羊FecB基因在新疆细毛羊胎儿成纤维细胞中的表达 |
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| 引用本文： | 于振兴,贺志锐,吾热力哈孜,刘强,孟仁,姚建龙,赛务加甫.湖羊FecB基因在新疆细毛羊胎儿成纤维细胞中的表达[J].动物医学进展,2012,33(6):99-102. |
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| 作者姓名： | 于振兴 贺志锐 吾热力哈孜 刘强 孟仁 姚建龙 赛务加甫 |
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| 作者单位： | 石河子大学动物科技学院,新疆石河子,832000 |
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| 摘 要： | 为培育携带FecB多胎基因的高繁殖率新疆细毛羊,本研究提取了湖羊卵巢的总RNA,在此基础上反转录为cDNA,通过PCR扩增得到了湖羊的FecB基因,再与pMD19-T载体连接,构建了pMD19-T-FecB重组质粒。通过双酶切与真核表达载体pEGFP-N1连接,得到了pEGFP-N1-FecB真核表达载体。经测序与酶切证明,成功构建了pEGFP-N1-FecB真核表达载体,并转染新疆细毛羊胎儿成纤维细胞,通过药物筛选获得了表达湖羊FecB基因的阳性新疆细毛羊胎儿成纤维细胞,为培育高繁殖率的新疆细毛羊奠定基础。
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| 关 键 词： | FecB基因 真核表达载体 胎儿成纤维细胞 湖羊 新疆细毛羊 |
Expression of Hu Sheep FecB Gene in Fetal Fibroblasts of Xinjiang Fine-wool Sheep |
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| YU Zhen-xing
,
HE Zhi-rui
,
Wureli Hazi
,
LIU Qiang
,
MENG Ren
,
YAO Jian-long
,
SAI Wujiafu.Expression of Hu Sheep FecB Gene in Fetal Fibroblasts of Xinjiang Fine-wool Sheep[J].Progress In Veterinary Medicine,2012,33(6):99-102. |
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| Authors: | YU Zhen-xing HE Zhi-rui Wureli Hazi LIU Qiang MENG Ren YAO Jian-long SAI Wujiafu |
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| Institution: | YU Zhen-xing1,HE Zhi-rui2,Wureli Hazi2,LIU Qiang2,MENG Ren2,YAO Jian-long2,SAI Wujiafu1(College of Animal Science and Technology,Shihezi University,Shihezi,Xingjiang,832000,China) |
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| Abstract: | To cultivate the Xinjiang fine-wool sheep with high reproduction rate,the ovarian total RNA of Hu sheep was extracted and reverse-transcribed into cDNA,and Hu sheep FecB gene was obtained by PCR amplification.Hu sheep FecB gene was linked to pMD19-T plasmid to construct the pMD19-T-FecB recombinant plasmid.pEGFP-N1-FecB eukaryotic expression vecor was constructed,and Hu sheep FecB gene was linked to pEGFP-N1 vector by double digestion.The sequencing and restriction enzyme digestion results showed that the pEGFP-N1-FecB eukaryotic expression vector was successfully constructed.The pEGFP-N1-FecB was thansfected into fetal fibroblast cells of Xinjiang fine-wool sheep and the positive cells was gotten by drug resistance.The cells gotten in this experiment can be used as the foundation to cultivate the Xinjiang fine-wool sheep with high reproduction rate. |
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| Keywords: | FecB gene eukaryotic expression vector fetal fibroblast Hu sheep Xinjiang fine-wool sheep |
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