| 广东省宠物源伪中间型葡萄球菌的耐药性调查研究 |
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| 引用本文： | 冯言言,田伟,罗倩怡,周颖泽,曾振灵,陈杖榴,刘健华.广东省宠物源伪中间型葡萄球菌的耐药性调查研究[J].动物医学进展,2012,33(6):6-10. |
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| 作者姓名： | 冯言言 田伟 罗倩怡 周颖泽 曾振灵 陈杖榴 刘健华 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 基金项目： | 高等学校博士学科点专项科研基金项目 |
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| 摘 要： | 为调查广东省宠物源伪中间型葡萄球菌(Staphylococcus pseudintermedius)的携带和耐药情况,从小动物临床采集皮肤、耳部和鼻腔等部位的样品,分离鉴定伪中间型葡萄球菌,并采用琼脂稀释法和纸片扩散法对伪中间型葡萄球菌进行抗菌药物敏感性检测,采用PCR方法检测mecA基因。结果表明,从898个样品中共分离到144株伪中间型葡萄球菌,检出率为16.0%,皮肤、耳部和鼻腔的伪中间型葡萄球菌检出率分别为20%(64/319)、17.8%(64/359)和7.5%(3/40)。抗菌药物敏感性检测和mecA基因检测结果显示有89株(61.8%)为耐甲氧西林伪中间型葡萄球菌(MRSP)。伪中间型葡萄球菌对氨苄西林、红霉素、阿奇霉素、克林霉素和四环素的耐药率均在80%以上,对利福平和阿米卡星的耐药率低于8%,对万古霉素、利奈唑胺、喹奴普汀/达福普汀和替考拉宁均敏感。大部分(>69%)MRSP对红霉素、克林霉素、泰乐菌素、阿奇霉素、复方新诺明、四环素、环丙沙星和恩诺沙星等抗菌药物表现耐药。本研究结果表明宠物源伪中间型葡萄球菌的耐药情况严重且MRSP的检出率高,应高度重视宠物源细菌耐药问题。
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| 关 键 词： | 耐甲氧西林伪中间型葡萄球菌 耐药 伪中间型葡萄球菌 宠物 |
Antimicrobial Resistance of Staphylococcus pseudintermedius Isolated from Pets in Guangdong Province |
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| FENG Yan-yan
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TIAN Wei
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LUO Qian-yi
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ZHOU Ying-ze
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ZENG Zhen-ling
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CHEN Zhang-liu
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LIU Jian-hua.Antimicrobial Resistance of Staphylococcus pseudintermedius Isolated from Pets in Guangdong Province[J].Progress In Veterinary Medicine,2012,33(6):6-10. |
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| Authors: | FENG Yan-yan TIAN Wei LUO Qian-yi ZHOU Ying-ze ZENG Zhen-ling CHEN Zhang-liu LIU Jian-hua |
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| Institution: | (College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China) |
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| Abstract: | The purpose of this study was to investigate the carriage rate and antimicrobial resistance of Staphylococcus pseudintermedius(SP) isolated from pets in Guangdong province.S.pseudintermedius were isolated from samples collected from skin,ear,nasal cavity and other parts of pets.The antimicrobial susceptibility test was carried out by agar dilution method and disk diffusion method.PCR was carried out to detect mecA gene.A total of 144(16.0%) S.pseudintermedius strains were obtained from the 898 samples collected from pets.The detection rates of skin,ear and nasal cavity were 20%(64/319),17.8%(64/359) and 7.5%(3/40),respectively.The antimicrobial susceptibility test results and mecA detection result showed that 89(61.8%) were methicillin-resistant S.pseudintermedius(MRSP).Resistance rates of the 144 S.pseudintermedius to ampicillin,erythromycin,azithromycin,clindamycin and tetracycline were over 80%.Resistance rates to amikacin and rifampicin was under 8%.All of the 144 S.pseudintermedius were susceptible to vancomycin,linezolid,quinupristin/dalfopristin and teicoplanin.Most(more than 69%) MRSP resisted to erythromycin,clindamycin,tylosin,azithromycin,trimethoprim-sulfamethoxazole,tetracycline,ciprofloxacin,and enrofloxacin.In conclusion,the high level antimicrobial resistance and high detection rate of MRSP was observed among the S.pseudintermedius isolates from companion animals.More attention should be addressed to the serious bacterial resistance in companion animals. |
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| Keywords: | MRSP antimicrobial resistance Staphylococcus pseudintermedius |
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