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灰葡萄孢拮抗细菌的筛选
引用本文:童蕴慧,徐敬友,陈夕军.灰葡萄孢拮抗细菌的筛选[J].中国生物防治,2000,16(3):123-126.
作者姓名:童蕴慧  徐敬友  陈夕军
作者单位:扬州大学农学院植物保护系,扬州,225009
基金项目:江苏省教委自然科学基金!(JW970 10 )
摘    要:采集扬州、淮阴两地16种常见植物根部土壤、根和叶片样本182份,分离纯化后得到细菌632株,抑菌圈法测定对灰葡萄孢有拮抗性的58株,拮抗性稳定的13株。其中拮抗性最强的Y2-11-1菌株为芽孢杆菌,抑菌圈直径达29.7mm。接种试验表明,Y2-11-1以番茄叶片和果实灰霉病的防效分别达68.7%和75.0%。部分菌株对其他重要植物病原真菌也有一定的拮抗作用。

关 键 词:灰葡萄孢  拮抗细菌  生物防治  番茄  病害
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Screening Antagonistic Bacteria Against Botrytis cinerea
TONG Yunhui,XU Jingyou,CHEN Xijun.Screening Antagonistic Bacteria Against Botrytis cinerea[J].Chinese Journal of Biological Control,2000,16(3):123-126.
Authors:TONG Yunhui  XU Jingyou  CHEN Xijun
Abstract:bacterial isolates were obtained from root soil system, root and leaf of 16 kinds of vegetables at Yangzhou and Huaiyin area of Jiangsu The bacterial isolates against Botrytis cinerea Pers ex Fr were screened by the inhibition zone method 58 isolates showed antagonistic effect on B cinerea and the inhibition zone diameter ranged from 6 5mm to 29 7mm The isolate Y 2 11 1 had the strongest and stable inhibiting activity against the pathogen and was identified as Bacillus sp The infection of the pathogen was decreased to 68 7%-75 0% by spraying the cell suspension of Y 2 11 1 and the pathogen suspension on the leaves and fruits of tomato respectively The inhibition of some isolates against other pathogens was investigated
Keywords:Botrytis cinerea  antagonistic bacteria  biocontrol
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