| 乙肝清胶囊中金丝桃素含量的测定 |
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| 引用本文： | 袁琳,雷磊,王敏智,辛秀,王萍,王兴.乙肝清胶囊中金丝桃素含量的测定[J].安徽农业科学,2008,36(25). |
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| 作者姓名： | 袁琳 雷磊 王敏智 辛秀 王萍 王兴 |
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| 作者单位： | 西南交通大学药学院,四川峨眉,614202;西南交通大学药学院,四川峨眉,614202;现代中药制剂教育部重点实验室,江西南昌,330004 |
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| 摘 要： | 目的]建立乙肝清胶囊中金丝桃素的HPLC含量测定方法。方法]筛选超声和加热回流提取2种方法在不同的提取溶媒下对胶囊内容物的前处理效果,采用HPLC法测定金丝桃素含量。色谱条件:色谱柱采用Phenomenex Luna C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-2.5 g/L KH2PO4水溶液(95∶5,V/V),流速为1.0 ml/min,检测波长为588 nm。结果]最优提取方法为:以甲醇为提取溶媒在90℃水浴加热回流30 min;金丝桃素在0.108~1.080μg范围内线性关系良好,r=0.999 84,平均回收率为98.8%,RSD=1.65%。结论]所建立的乙肝清胶囊中金丝桃素HPLC含量测定方法具有良好的稳定性、重现性和准确性,可用于乙肝清胶囊的质量控制。
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| 关 键 词： | 金丝桃素 测定 乙肝清胶囊 高效液相色谱法 |
Determination of Hypericin in Yiganqing Capsule by HPLC |
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| YUAN Lin et al.Determination of Hypericin in Yiganqing Capsule by HPLC[J].Journal of Anhui Agricultural Sciences,2008,36(25). |
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| Authors: | YUAN Lin |
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| Abstract: | Objective] The research aimed to establish HPLC method for determination of hypericin in Yiganqing capsule.Method] Two different methods of ultrasonic extraction and heating reflux extraction were applied to extract hypericin from the capsule's content in different solvents.The extraction effects were evaluated through the determination of hypericin by HPLC to find out the optimum extraction condition.HPLC conditions were as follows: Phenomenex Luna C18(4.6 mm×250 mm,5 μm) as analytic column, methanol-2.5 g/L KH2PO4(95∶5,V/V) as mobile phase,the detection wavelength was set at 588 nm,and the flow rate was 1.0 ml/min.Result] The optimum extraction method was that,the Yiganqing capsule's content was extracted with methanol for 30 minutes by heating reflux at 90 ℃ in a water bath.The linearity of hypericin was good at the range of 0.108-1.080 μg,r=0.999 84,and the mean value of recovery was 98.8% with RSD of 1.65%.Conclusion] HPLC method was established for determination of hypericin in Yiganqing capsule with good stability and accuracy.It could be used for the quality control of Yiganqing capsule. |
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| Keywords: | Hypericin Determination Yiganqing capsule HPLC |
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