| 高致病性猪繁殖与呼吸综合征病毒(PRRSV)气溶胶的发生与传播机制 |
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| 引用本文： | 曹永芝,马卫明,陈书民,柴同杰,宋移福,贺加双,刘莲莲,谢冰.高致病性猪繁殖与呼吸综合征病毒(PRRSV)气溶胶的发生与传播机制[J].中国兽医学报,2010,30(2). |
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| 作者姓名： | 曹永芝 马卫明 陈书民 柴同杰 宋移福 贺加双 刘莲莲 谢冰 |
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| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018
2. 山东省畜牧兽医总站检验中心,山东,济南,250022 |
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| 基金项目： | 博士后基金资助项目(76211); 山东省自然科学基金资助项目(Y2006D14); 山东省博士后创新项目专项资金资助项目(200703043) |
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| 摘 要： | 为深入认识猪繁殖与呼吸综合征病毒(PRRSV)气溶胶的发生与传播机制,将35日龄无PRRSV抗体仔猪预饲1周后分为4组(PRSSV攻毒组、直接接触组、间接接触组和阴性对照组),饲养于正负压隔离器中。2个隔离器置于不同室内并通过管道相连,采用AGI-30收集器收集其空气样品。然后,将其接种Marc-145细胞,再用RT-PCR检测病毒;并检测血常规和抗体变化。结果发现,攻毒组于攻毒后4d开始形成气溶胶,并持续到试验结束。气溶胶高峰出现在攻毒后15d。气溶胶传播的间接接触组从临床症状、病理变化、提取病理组织核酸和抗体检测等多个方面都证明其感染了PRRSV,并经鼻腔向外排毒;直接接触组和间接接触组几乎同时感染PRRSV。试验表明,PRRSV不仅能形成气溶胶并且能够通过其迅速感染临近猪群。
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| 关 键 词： | 仔猪 高致病性猪繁殖与呼吸综合征病毒 气溶胶 传播机制 |
Transmission mechanism of porcine reproductive and respiratory syndrome virus aerosol |
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| CAO Yong-zhi,MA Wei-ming,CHEN Shu-min,CHAI Tong-jie,SONG Yi-fu,HE Jia-shuang,LIU Lian-lian,XIE Bing.Transmission mechanism of porcine reproductive and respiratory syndrome virus aerosol[J].Chinese Journal of Veterinary Science,2010,30(2). |
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| Authors: | CAO Yong-zhi MA Wei-ming CHEN Shu-min CHAI Tong-jie SONG Yi-fu HE Jia-shuang LIU Lian-lian XIE Bing |
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| Abstract: | The porcine reproductive and respiratory syndrome-free piglets were bred in a positive and negative pressure isolator which were connected by aleak-free pipe.Aerosol samples were collected by AGI-30 (All Glass Impinger-30) extractor,simultaneously nasal swabs,whole blood and serum samples were collected in different periods after challenged with PRRSV.The above-mentioned samples were detected by cell culture and RT-PCR.The results indicated that aerosols could be isolated from 4 d to the last day of the exp... |
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| Keywords: | piglet high virulent porcine reproductive and respiratory syndrome virus aerosol transmission mechanism |
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