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Internal transcribed spacer sequences of nuclear ribosomal DNA resolving complex taxonomic history in the genus Vicia L. |
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| Authors: | Behrouz Shiran Soghra Kiani Deepmala Sehgal Akram Hafizi Tanvir ul-Hassan Manju Chaudhary Soom Nath Raina |
| Institution: | 1. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Shahrekord University, 8818634141, Shahrekord, Iran 2. Department of Agriculture, Payame Noor University, PO Box 19395-3697, Tehran, Iran 3. International Wheat and Maize Improvement Center (CIMMYT), Km. 45, Carretera, Mex-Veracruz, El Batan, CP 56130, Texcoco, Mexico 4. Department of Botany, University of Delhi, Delhi, 110007, India 5. Amity Institute of Biotechnology, Amity University, Sector 125, Noida, U.P., India |
| Abstract: | The ITS1-5.8S-ITS2 region of the 18S-28S nrDNA was sequenced in 49 Vicia species and subspecies selected from nine sections of the two subgenera to resolve taxonomic contradictions. Phylogenies derived from the internal transcribed spacer (ITS) sequences strongly support the view that both Vicia faba and Vicia bithynica are distant from the Narbonensis species complex (NSC) in section Faba. V. faba is more closely aligned with sections Peregrinae and Hypechusa, whereas V. bithynica is more closely aligned with section Vicia. Six synapomorphic substitutions unique to the NSC support a separate section for the Narbonensis complex. Vicia johannis subsp. johannis was the most diverged species within the NSC with high genetic distance between V. johannis subsp. johannis and remaining species and subspecies of the NSC. These results suggest that Vicia johannis subsp. johannis should be given species rank. The neighbor-joining and parsimony based trees supported the sections Vicia (after the exclusion of Vicia lathyroides) and Cracca (after the exclusion of Vicia vicioides and Vicia hirsuta). The section Hypechusa is polyphyletic and paraphyletic based on the present ITS sequence cladistic analysis. The x = 7 as the ancestral number for the genus is supported by the present study. |
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