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| 中草药提取物对板蓝根根腐病菌抑制作用研究 | |
| 引用本文： | 李红梅,曹静,张凤巧,客绍英,王树桐.中草药提取物对板蓝根根腐病菌抑制作用研究[J].河南农业科学,2009(6). |
| 作者姓名： | 李红梅 曹静 张凤巧 客绍英 王树桐 |
| 作者单位： | 1. 河北农业大学,植物保护学院,河北省生物防治工程技术研究中心,河北,保定,071001 2. 唐山师范学院,河北,唐山,063000;聊城大学,农学院,山东,聊城,252059 3. 唐山师范学院,河北,唐山,063000 |
| 基金项目： | 河北省教育厅科学计划项目，唐山市科技局重点实验室项目，河北农业大学科技创新基金 |
| 摘 要： | 测试了123种中草药提取物对茄腐镰刀菌(Fusarium solani)菌落生长及分生孢子萌发的抑制作用。结果表明:提取物浓度为0.01 g/mL时,99种提取物对菌落生长具有显著的抑制作用,其中苦楝皮和威灵仙的抑菌率大于50%,北沙参的抑菌率达到了100%;112种提取物对分生孢子萌发具有显著抑制作用,其中11种提取物的抑制率在50%以上,而且北沙参和牛膝的抑制率达到了100%。将抑制率大于50%的11种提取物稀释为200倍,进行二次筛选,发现苦楝皮和威灵仙2种提取物对菌落生长的抑制作用下降,而北沙参对菌落生长抑菌率仍为100%;11种提取物对分生孢子萌发抑制率下降的幅度在21.59%~47.02%,而北沙参的抑制率最高,为74.50%。北沙参的抑制中浓度(EC50)为3.621 mg/mL。 |
| 关 键 词： | 板蓝根 根腐病 中草药提取物 抑菌率 |
Inhibitory Effect of Chinese Medicinal Herb Extract on the Pathogen Causing Root Rot Disease of Isatis indigotica Fort |
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| LI Hong-mei,CAO Jing,ZHANG Feng-qiao,KE Shao-ying,WANG Shu-tong.Inhibitory Effect of Chinese Medicinal Herb Extract on the Pathogen Causing Root Rot Disease of Isatis indigotica Fort[J].Journal of Henan Agricultural Sciences,2009(6). | |
| Authors: | LI Hong-mei CAO Jing ZHANG Feng-qiao KE Shao-ying WANG Shu-tong |
| Abstract: | Fusarium solani was confirmed as the main causal agent of Isatis indigotica Fort root rot disease.Inhibitory effect of 123 species of Chinese medicinal herb extracts(CMHE) was tested against the colony growth and conidial germination of F.solani.The results indicated that ninety-nine species of Chinese herbs had significant inhibitory effect against the colony growth with the concentration of 0.01 g/mL.For example,the inhibition ratio of Cortex meliae and Clematis chinensis were over 50%,and Glehnia littoralis reached 100%.One hundred and thirteen species of Chinese herbs had significant inhibitory effect against conidial germination,eleven of them were over 50% inhibition,and the inhibition rates of Glehnia littoralis and Radix achyranthis bidentatae were 100%.These extracts with inhibition rates over 50% were chosen for the secondary screen at the concentration of 0.005g/mL.Glehnia littoralis had 100% inhibitory effect in the second screening tests,while the inhibition ratio of Cortex meliae and Clematis chinensis against colony growth decreased significantly.The inhibition ratio of eleven extracts against coni-dial germination reduced by 21.59%-47.02%.The inhibition rate of Glehnia littoralis was 74.50%,which was the best one with the EC50 3.621 mg/mL. |
| Keywords: | Isatis indigotica Fort Root rot disease Chinese herbs extracts Inhibitory effect |
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