Proteomics Analysis of Gossypium barbadense Leaves with Resistance to Verticillium dahliae under Infection Stress |
| |
| Authors: | Xu Qi Wu Linlin Wang Mi Li Xiaoping Guo Wenzhi Pei Lei |
| |
| Institution: | 1. Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000, China; 2. Shanxi Key Laboratory of Cotton Germplasm Resources Utilization and Molecular Design Breeding, Yuncheng, Shanxi 044000, China; 3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China |
| |
| Abstract: | Objective] The purpose of this study is to analyze the resistance mechanism of island cotton under Verticillium wilt stress and to find possible resistance genes. Method] The cotton resistance mechanism under the stress of Verticillium dahliae was studied by protein two-dimensional electrophoresis and mass spectrometry at the protein level. Result] In the leaves, eleven proteins were down-regulated and 15 proteins were up-regulated after 2 hours of inoculation with Verticillium dahliae. The down-regulated proteins were mainly related to photosynthesis and carbon assimilation, and then we deduce that Verticillium dahliae is mainly broken cotton photosynthetic system to cause cotton susceptibility. The up-regulated proteins were mainly related to photosynthesis and benzoquinone reductase, beta-D-galactosidase, 14-3-3f protein and other disease-resistant protein. Conclusion] It is speculated that the defense mechanism of island cotton on Verticillium wilt may occur at two levels: one is passive defense, it is manifested that the isoproteins of the down-regulated proteins such as chloroplast II AB binding protein and ribulose diphosphate carboxylase are highly expressed to maintain the stability of the island cotton photosynthetic system, the histone and 14-3-3f protein are highly expressed to keep the cell stability; the second is active defense, it is manifested that the high expressed β-D-galactosidase and benzoquinone reductase may be involved in the resistance of island cotton to Verticillium wilt. |
| |
| Keywords: | cotton Gossypium barbadense Verticillium wilt Verticillium dahliae proteomes expression profile |
|
| 点击此处可从《棉花学报》浏览原始摘要信息 |
| 点击此处可从《棉花学报》下载免费的PDF全文 |
|
