| 美国鲥鱼内脏结节病的组织病理学观察 |
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| 引用本文： | 徐金铖,刘宇婷,潘连德,杨吉平.美国鲥鱼内脏结节病的组织病理学观察[J].上海海洋大学学报,2021,30(2):247-257. |
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| 作者姓名： | 徐金铖 刘宇婷 潘连德 杨吉平 |
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| 作者单位： | 上海海洋大学,上海海洋大学,上海海洋大学,上海沁淼生物科技有限公司 |
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| 基金项目： | 水产动物遗传育种上海市协同创新中心（ZF1206） |
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| 摘 要： | 为了探究美国鲥鱼内脏结节病的病因病理和防控办法,本实验从患有内脏结节病的美国鲥鱼体内分离出肝、脾和肾等内脏器官组织,通过对病鱼样品H.E常规染色、马松染色和抗酸杆菌染色,并结合透射电镜对病变组织、细胞以及病原菌进行观察分析。病鱼的临床症状表现为体表多处损伤,鳞片下出血,腹部轻微膨大,肛门按压出血。病理解剖发现腹腔内含大量血液混合腹水,肝脏、肾脏、脾脏等器官有大量白色结节,肝脏表面呈深红色,多处破裂出血,肾脏表面附着大量白色纤维样病变,失去肾脏原有颜色和结构,其他器官未见结节,肠道出血。组织病理分析结果表明：显微结构所见肝、脾和肾器官组织病变是出现大量肉芽肿,且又大又多。肉芽肿中心由坏死的组织碎片和聚生菌体组成,外围有大量的炎性细胞浸润,包膜由纤维组织构成。肝脏组织内含有大量空泡为脂肪变性；肾组织内肾小管腔破裂分解,失去原有基本结构,肾间质出现大面积组织坏死和崩解,部分坏死组织呈现干酪样变性；脾组织间充斥大量的红细胞。其它内脏器官组织内未见肉芽肿。抗酸染色和透射电镜观察证实一种短杆菌感染形成肉芽肿。由此得出,肝、脾和肾组织由于病原菌感染形成大量肉芽肿继而导致脏器产生大量的结节,最终出现器官功能低下或衰竭导致鱼体死亡。
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| 关 键 词： | 美国鲥鱼 结节病 组织病理 肉芽肿 |
| 收稿时间： | 2020/2/21 0:00:00 |
| 修稿时间： | 2020/5/29 0:00:00 |
Histopathological observation of visceral sarcoidosis in Alosa sapidissima |
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| XU Jincheng,LIU Yuting,PAN Liande,YANG Jiping.Histopathological observation of visceral sarcoidosis in Alosa sapidissima[J].Journal of Shanghai Ocean University,2021,30(2):247-257. |
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| Authors: | XU Jincheng LIU Yuting PAN Liande YANG Jiping |
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| Institution: | Shanghai Ocean University,Shanghai Ocean University,Shanghai Ocean University,Shanghai Qinmiao Biological Technology Co., Ltd. |
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| Abstract: | In order to investigate the etiology, pathology and method of prevention and cure of visceral sarcoidosis in American shad(Alosa sapidissima), The liver, spleen, kidney and other internal organ tissues from American shad were isolated diseased tissues, cells and pathogenic bacteria were observed and analyzed by H.E routine staining and Ziehl-Neelsen staining, combined with transmission electron microscopy. The clinical symptoms of the sick American shad were multiple injuries on the body surface, bleeding under the scales, slightly enlarged abdomen and bleeding from the anus. Pathological anatomy revealed that the abdominal cavity contained a large amount of blood mixed with ascites, and there were a great number of white nodules in internal organs such as liver, kidney and spleen. With multiple ruptures and bleeding, the liver surface was dark red, the surface of the kidney was covered with numerous white fibrous lesions, and the original color and structure of the kidney were changed. Nodules were not seen in other organs, and bleeding was observed in the intestines. The results of histopathological analysis showed that many granulomas formed in the liver, spleen, and kidney organ tissues. The granuloma center consisted of necrotic tissue fragments and concomitant bacteria, the periphery was infiltrated with a large number of inflammatory cells, and the envelope was composed of fibrous tissue. Liver tissue contained many vacuoles, which were steatosis. Renal tubules in kidney tissue ruptured and decomposed, losing the original basic structure, large area of ??tissue necrosis and disintegration appeared in renal interstitial tissue, and part of necrotic tissue showing caseous degeneration. Spleen tissue was filled with a large proportion of red blood cells. No granuloma was found in other internal organs. Obversation with Ziehl-Neelsen staining and transmission electron microscopy confirmed that a brevibacterium infection formed granulomas. The results indicated that a large number of granulomas formed in liver, spleen, and kidney tissues because of pathogens infection, which led to numerous nodules in the organs. Eventually, organ dysfunction or failure resulted in death of disabled fish. |
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| Keywords: | Alosa sapidissima sarcoidosis histopathology granuloma |
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