| 环境DNA技术在象山港水域鱼类多样性调查中的应用与评估 |
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| 引用本文： | 凌建忠,姜亚洲,孙鹏,袁兴伟,张辉,唐保军.环境DNA技术在象山港水域鱼类多样性调查中的应用与评估[J].中国水产科学,2021,28(2):205-214. |
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| 作者姓名： | 凌建忠 姜亚洲 孙鹏 袁兴伟 张辉 唐保军 |
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| 作者单位： | 中国水产科学研究院东海水产研究所, 农业农村部东海与远洋渔业资源开发利用重点实验室, 上海 200090 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目(201303047)； 中国水产科学研究院基本科研业务费(2019ZD1102). |
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| 摘 要： | 通过对象山港水域环境DNA(eDNA)样品的采集和高通量测序分析,并结合渔业资源调查数据,阐述象山港主要鱼类群落的种类组成和多样性特征,探讨了环境DNA技术在典型海域鱼类多样性研究中的应用前景.结果显示,共从象山港水域环境DNA样品中检测到26个常见鱼类物种,隶属于辐鳍鱼纲(Actinopterygii)的7个目中的2...
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| 关 键 词： | 环境DNA(eDNA) 象山港 渔业资源 高通量测序 鱼类多样性 |
Application and evaluation of environmental DNA technology in fish diversity research in Xiangshan Bay |
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| LING Jianzhong,JIANG Yazhou,SUN Peng,YUAN Xingwei,ZHANG Hui,TANG Baojun.Application and evaluation of environmental DNA technology in fish diversity research in Xiangshan Bay[J].Journal of Fishery Sciences of China,2021,28(2):205-214. |
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| Authors: | LING Jianzhong JIANG Yazhou SUN Peng YUAN Xingwei ZHANG Hui TANG Baojun |
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| Abstract: | Fish are one of the key groups of organisms in marine ecosystems and are also the main object of marine fisheries. Knowledge of their diversity and distribution not only provides basic data for the sustainable development and utilization of marine ecosystems but also set up a theoretical basis for evaluating the influence of human disturbance factors (such as marine development and fishing) on these ecosystems. With the rapid development of biotechnology in recent years, environmental DNA (eDNA) technology has been gradually applied in the field of ecology owing to its advantages in sensitivity, accuracy, and cost, but there have been few local surveys of marine fish resources and their diversity using eDNA, especially in the local marine areas and fishing grounds. Xiangshan Bay is a semi-closed narrow harbor that is located on the north coast of the central Zhejiang Province, China. For years, overfishing, environmental pollution, and large-scale marine development activities have caused the ecological environment to deteriorate, and fishery resources have declined. Biological resource studies in Xiangshan Bay are still insufficient, and research on fish community structure and diversity are lacking. However, owing to the existence of many artificial and natural reefs, fish trawling surveys cannot be carried out in the area, which also limits the routine detection of fish biodiversity (especially for reef-dwelling fish) in the area. Therefore, the application of eDNA technology is of great significance to the assessment of fish biodiversity in Xiangshan Bay. In the present study, by eDNA sample collection and high-throughput sequencing analysis, combined with survey data of fishery resources in the same region, the fish species composition and diversity characteristics of the main fish communities in Xiangshan Bay were expounded. The prospect of applying eDNA technology in fish diversity studies of typical sea areas was also discussed. The results showed that 26 common fish species (belonging to seven orders of Actinopterygii and from 21 genera) were detected from the aquatic eDNA samples from Xiangshan Bay. Two orders with the highest abundance were Clupeiformes and Perciformes, whose relative abundance accounted for 92.5% of the total fish species abundance. Among all the fish species identified from eDNA analysis, Konosirus punctatus and Acanthopagrus schlegelii had the highest abundance, which accounted for 45.85% and 17.69% of the total fish abundance, respectively. Both species also contributed the most to fisheries resources in Xiangshan Bay. The next most abundant species were Tridentiger barbatus and Lateolabrax japonicus. The sequence abundances of such taxa were similar to the trends in the fisheries catch data. Compared with traditional survey methods, the eDNA analysis had high sensitivity, high accuracy, and low cost and was suitable for studying the fish diversity of relevant sea areas. The present research not only enriched the structural and functional information of the Xiangshan Bay aquatic ecosystem but also provided basic information to support the management and restoration of marine ecosystems in this marine area. |
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