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ELISA快速检验饲料和家畜尿液中的“瘦肉精”
引用本文:武晓宏,刘旭,杨俊华.ELISA快速检验饲料和家畜尿液中的“瘦肉精”[J].中国动物保健,2012(4):18-21.
作者姓名:武晓宏  刘旭  杨俊华
作者单位:1. 宁夏兽医饲料监察所 宁夏银川 750011
2. 宁夏银川宏佳兽药店 宁夏银川 750004
基金项目:山东省科学技术发展计划项目(2007GG1QX09017)
摘    要:酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA),是目前检测家畜饲料、产品以及组织、尿液中瘦肉精的最常用、最快速的方法。本文针对此方法和其它检测方法做了详细的比较,提出了酶联免疫吸附方法点板操作时的注意事项,并总结了在检测样品过程中注意的要点和经验。

关 键 词:酶联免疫吸附法(ELISA)  气相色谱-质谱法(GC-MS)  高效液相色谱法(HPLC)  瘦肉精  β-兴奋剂

ELISA in Rapid Testing of Clenbuterol in the Feed and Livestock Urine Liquid
Wu Xiaohong,Liu Xu,Yang Junhua.ELISA in Rapid Testing of Clenbuterol in the Feed and Livestock Urine Liquid[J].China Animal Health,2012(4):18-21.
Authors:Wu Xiaohong  Liu Xu  Yang Junhua
Institution:1.Feed monitoring of veterinary drugs in Ningxia;2.Ningxia yinchuan hongjia pharmacies Ningxia yinchuan 750004)
Abstract:Enzyme-linked immunosorbent assay(ELISA) is the most rapid test method be used to detect the lean fine currently from livestock feed,products and organization,as well as urine liquid.Based on the comparison of this method and other detection approach,we put forward a detailed comparison,proposed enzyme immunoassay method considerations for Board action,and summarizes the main points of note in the test sample processes and experience.
Keywords:enzyme-linked immunosorbent assay(ELISA)  gas chromatography-mass spectrometry(GC-MS)  high-performance liquid chromatography(HPLC)  clenbuterol  β-stimulant
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