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| 外源DNA导入小麦后雄性不育变异的初步研究 | |
| 引用本文： | 杨景成,于元杰,齐延芳.外源DNA导入小麦后雄性不育变异的初步研究[J].核农学报,2004,18(1):6-10. |
| 作者姓名： | 杨景成 于元杰 齐延芳 |
| 作者单位： | 1. 北京自然博物馆,北京,100093 2. 山东农业大学农学院,山东,泰安,271018 3. 山东省农业科学院原子能农业应用研究所,山东,济南,250100 |
| 基金项目： | 山东省聊城市国家大型优质小麦基地项目及国家科技部和山东省科技厅"九五"重点项目资助(96-C01-01-02-12) |
| 摘 要： | 利用浸泡法将外源λDNA导入普通小麦品系 81 45 2 7,获得不育变异株 ,稳定为不育系 ,简称D型不育系。该不育系小花结构具有显著特点 ,多数小花子房数目增加 ,花药数目减少 ,而且二者之和并非恒定为 4。多子房小花一般位于穗的中部 ,而顶部和基部小花未发现多子房现象。多子房小花的主子房位于小花的中央 ,副子房着生于主子房的周围。子房的发育进程不同步 ,主子房发育较早 ,体积较大 ;副子房发育较慢 ,而且未发育成熟即退化萎缩。花药多呈短棒状、月牙状等畸形状态 ,且多数在早期即退化萎缩 ,无生活力花粉。杂交试验表明 ,该不育系为细胞质雄性不育系 ,不育度达 99% ,受体品系 81 45 2 7可作其保持系。不育系与鲁麦 1 4号的杂种一代在穗长、每穗小穗数和穗粒数等性状上存在一定程度的杂种优势 ,单株产量较鲁麦 1 4号增加 1 1 6%。 |
| 关 键 词： | 小麦 外源DNA导入 细胞质雄性不育 花结构异常 |
| 文章编号： | 1000-8551(2004)01-006-05 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
PRELIMINARY STUDIES ON MALE STERILE MUTANT AFTER EXOGENOUS DNA INTRODUCED INTO WHEAT |
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| YANG Jing-cheng,YU Yuan-jie,QI Yan-fang.PRELIMINARY STUDIES ON MALE STERILE MUTANT AFTER EXOGENOUS DNA INTRODUCED INTO WHEAT[J].Acta Agriculturae Nucleatae Sinica,2004,18(1):6-10. | |
| Authors: | YANG Jing-cheng YU Yuan-jie QI Yan-fang |
| Institution: | YANG Jing-cheng 1 YU Yuan-jie 2 QI Yan-fang 3 |
| Abstract: | A stable sterile line—D-type sterile line was obtained after exogenous λDNA introduced into wheat line 814527. There were obvious characters in flower structure of the sterile line. The number of ovaries increased and that of anthers declined, and the sum was not always equal to four. Polygynous flowers were located in the middle of panicles, while there were no polygynous ones on the top and the base of panicles. The main ovary was in the center of the flower, and the accessory ovaries surrounded the main ovary. The ovaries did not develop synchronously, main ovary developed earlier and accessory ones slower. The volume of main ovary was bigger than the accessory ones. Anthers of the sterile line with abnormal shapes were obsolescent at early stage, and had no living pollen. It showed that the sterile line was a cytoplasmic male sterile one, and the acceptor line 814527 could be its maintainer. The hybrid of the sterile line and Lumai 14 had some heterosis in traits such as panicle length, spikelet number per panicle and grain number per panicle, and its yield per plant was 11.6% higher than that of Lumai 14. |
| Keywords: | wheat (Triticum aestivum L ) exogenous DNA introduction cytoplasmic male sterility structural abnormity of flower |
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