| 酶联免疫法测定鸡肉中尼卡巴嗪残留 |
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| 引用本文： | 王鹤佳.酶联免疫法测定鸡肉中尼卡巴嗪残留[J].中国兽药杂志,2014,48(3):59-61. |
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| 作者姓名： | 王鹤佳 |
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| 作者单位： | 中国兽医药品监察所 |
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| 基金项目： | 国家科技支撑计划（2011BAK10B01-11） |
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| 摘 要： | 为了建立鸡肉中尼卡巴嗪残留标志物4，4’-二硝基均二苯脲（DNC）残留酶联免疫（ELISA）检测方法。将N-琥珀酰-L-丙氨酰-L-丙氨酰-L-丙氨酸4-硝基苯胺（SAN）与载体蛋白偶联作为抗原免疫动物，获得抗DNC抗体，在此基础上建立了鸡肉中尼卡巴嗪残留的检测方法。人工抗原中SAN与载体蛋白的结合比约为12：1，血清效价1：2000倍，鸡肉中检测限为9．2μg／kg，添加回收率在49．4％～118％之间，批内、批间变异系数均小于20％。该方法在灵敏度、精密度和准确度方面均能满足兽药残留筛选检测要求。
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| 关 键 词： | 尼卡巴嗪 4 4’-二硝基均二苯脲 残留 鸡肉 酶联免疫法 |
| 收稿时间： | 2013/12/5 0:00:00 |
| 修稿时间： | 2014/2/27 0:00:00 |
Enzyme-linked immunosorbent assay for detection of nicarbazin residue in chicken muscle |
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| WANG He-jia,LIU Zhi-hong,WANG Xia,HUANG Yao-ling,SUN Lei.Enzyme-linked immunosorbent assay for detection of nicarbazin residue in chicken muscle[J].Chinese Journal of Veterinary Drug,2014,48(3):59-61. |
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| Authors: | WANG He-jia LIU Zhi-hong WANG Xia HUANG Yao-ling SUN Lei |
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| Institution: | China Institute of Veterinary Drug Control |
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| Abstract: | ELISA for detection of 4, 4 ＇ - dinitrocarbanilide （ DNC ） , marker residue of nicarbazin, in chicken muscle was developed. Anti-DNC antibody was gotten by immunizing animals with SAN-protein combination. The conjugate rate of SAN and protein was 12 ： 1 in artificial antigen. Antiserum valence was more than 1 ： 2000. The limit of detection was 9.2 μg/kg in chicken muscle. The recovery rate range was from 49.4% to 118%. The sensibility, precision and accuracy of the method could satisfy the request of screening veterinary drug residue |
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| Keywords: | nicarbazin DNC residue chicken muscle ELISA |
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