| 稻瘟病标样中分离真菌的分析和鉴定 |
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| 引用本文： | 俞咪娜,王伟舵,于俊杰,尹小乐,聂亚锋,刘永锋.稻瘟病标样中分离真菌的分析和鉴定[J].植物病理学报,2014,44(6):573-580. |
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| 作者姓名： | 俞咪娜 王伟舵 于俊杰 尹小乐 聂亚锋 刘永锋 |
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| 作者单位： | 江苏省农业科学院植物保护研究所,南京 210014 |
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| 基金项目： | 江苏省农业自主创新基金(CX(12)1003-10);国家科技支撑计划(2012BAD19B03) |
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| 摘 要： | 通过分离稻瘟病标样中的病原真菌,回接稻瘟病菌普感的水稻品种—丽江新团黑谷,得到多个不能引发稻瘟病的菌株。随机选取2个致病的分离菌株和4个不致病的分离菌株,以稻瘟病菌Guy11为对照,观察它们的菌落形态和色泽、分生孢子形态和大小、分生孢子梗形态等生物学特性,发现6个分离菌株与Guy11的形态均较为相近。附着胞形成试验发现,不致病的分离菌株在疏水表面不能形成附着胞,但外源添加cAMP可使部分孢子形成附着胞。PCR扩增分离菌株的ITS、β-tubulin、actin和calmodulin等序列,测序并根据比对结果绘制相应的系统发育树,鉴定表明分离的2个致病菌株9-1、214属于Magnaporthe oryzae;4个不致病菌株18-1、18-2、122和132为梨孢属真菌Pyricularia。不致病菌株的发现和鉴定可为稻瘟病菌侵染机制研究和稻瘟病防治奠定基础。
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| 关 键 词： | 稻瘟病菌 梨孢属 致病性 聚类分析 |
| 收稿时间： | 2013-08-07 |
Analysis and identification of the strains isolated from rice blast specimens |
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| YU Mi-na,WANG Wei-duo,YU Jun-jie,YIN Xiao-le,NIE Ya-feng,LIU Yong-feng.Analysis and identification of the strains isolated from rice blast specimens[J].Acta Phytopathologica Sinica,2014,44(6):573-580. |
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| Authors: | YU Mi-na WANG Wei-duo YU Jun-jie YIN Xiao-le NIE Ya-feng LIU Yong-feng |
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| Institution: | Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | Some monoconidial isolates, which were obtained from rice blast specimens, are non-pathogenic to LTH, a native variety in Yunnan without any major resistance genes. Two pathogenic isolates and four non-pathogenic isolates were randomly chosen and analyzed. Colony morphology, conidia and conidiophores of these six isolates were all similar to those of the Magnaporthe oryzae strain Guy11. However, the four non-pathogenic isolates could not develop appressoria on hydrophobic surfaces, but these could be partially rescued by exogenous cAMP. Phylogenetic analysis of the isolates were inferred using four sequences: ITS and portion of genes β-tubulin, actin and calmodulin. The results showed that two pathogenic isolates 9-1, 214 belonged to M. oryzae, and four non-pathogenic isolates 18-1, 18-2, 132 and 122 belonged to Pyricularia. Analysis and identification of the non-pathogenic strains can provide basic materials for investigating the infection mechanisms of rice blast fungus and developing novel mechanisms to control rice blast. |
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| Keywords: | Magnaporthe oryzae Pyricularia pathogenicity cluster analysis |
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