| 猪繁殖与呼吸综合征病毒传统毒株与变异毒株的鉴别诊断 |
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| 引用本文： | 于美芳,吴家强,张金强,李俊,张秀美,景田军,王雪红,王金宝.猪繁殖与呼吸综合征病毒传统毒株与变异毒株的鉴别诊断[J].家畜生态,2009(2). |
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| 作者姓名： | 于美芳 吴家强 张金强 李俊 张秀美 景田军 王雪红 王金宝 |
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| 作者单位： | 青岛农业大学动物科技学院;山东省农业科学院畜牧兽医研究所;山东牧工商总公司;山东出入境检验检疫局; |
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| 基金项目： | 山东省自然科学基金重点项目(Z2007D06);;山东省重大科技专项(2007ZHZX11103);;山东省农科院青年基金项目(2005YQ039) |
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| 摘 要： | 从山东各地猪场采集了50份疑似猪繁殖与呼吸综合征(PRRS)感染的病料,采用RT-PCR技术扩增出PRRS病毒(PRRSV)的ORF7基因和Nsp2基因,并对Nsp2基因进行了序列同源性比较和遗传进化树分析,从RT-PCR阳性病料中进行病毒分离,然后应用Nsp2单克隆抗体对分离毒株进行鉴别诊断。结果表明,从8份病料中同时扩增出病毒的ORF7基因和Nsp2基因,序列分析显示其中6株病毒的Nsp2缺失30个氨基酸,与Nsp2单克隆抗体不发生反应,属于变异毒株;另外两株病毒的Nsp2未见氨基酸缺失,与Nsp2单克隆抗体发生特异性反应,属于传统毒株。序列同源性和系统进化分析表明,6株变异毒株与其他高致病性毒株亲缘关系很近,而与经典毒株的遗传距离较远。
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| 关 键 词： | 猪 繁殖与呼吸综合征病毒 变异株 传统株 鉴别诊断 |
Differentiated Detection of Conventional from Variant Isolates on Porcine Reproductive and Respiratory Syndrome Virus |
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| YU Mei-fang,WU Jia-qiang,ZHANG Jin-qiang,LI Jun,ZHANG Xiu-mei,JING Tian-jun,WANG Xue-hong,WANG Jin-bao.Differentiated Detection of Conventional from Variant Isolates on Porcine Reproductive and Respiratory Syndrome Virus[J].Ecology of Domestic Animal,2009(2). |
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| Authors: | YU Mei-fang WU Jia-qiang ZHANG Jin-qiang LI Jun ZHANG Xiu-mei JING Tian-jun WANG Xue-hong WANG Jin-bao |
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| Institution: | 1.College of Animal Science and Technology;Qingdao Agricultural University;Qingdao;Shandong 266109;China;2.Institute of Animal Science and Veterinary Medicine;Shandong Academy of Agricultural Sciences;Jinan;Shandong 250100;3.Mu Gongshang Corporation Shandong;4.Shandong Exit Inspection and Quarantine;Shandong 266001;China |
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| Abstract: | A total of 50 samples which were suspected with porcine reproductive and respiratory syndrome were collected(PRRS) in Shandong area.The ORF7 gene and Nsp2 gene of PRRS virus(PRRSV) were amplified by RT-PCR,and nucleotide homolog and phylogenetic analysis were performed based on the Nsp2 gene of PRRSV.Viruses were isolated from PCR-positive samples and differentiation between conventional and variant PRRSV was conducted using monoclonal antibody(MAb) against Nsp2.It showed that ORF7 gene and Nsp2 gene were a... |
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| Keywords: | porcine reproductive and respiratory syndrome variant isolates conventional isolates differentiated detection |
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