| 尼罗罗非鱼8个养殖群体遗传差异的微卫星分析 |
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| 引用本文： | 李先仁,李思发,唐首杰,蔡完其.尼罗罗非鱼8个养殖群体遗传差异的微卫星分析[J].上海水产大学学报,2009,18(1). |
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| 作者姓名： | 李先仁 李思发 唐首杰 蔡完其 |
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| 作者单位： | 上海海洋大学农业部水产种质资源与养殖生态重点开放实验室,上海,201306 |
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| 基金项目： | 国家"十五"攻关项目 |
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| 摘 要： | 利用18对微卫星引物对尼罗罗非鱼(Oreochromis niloticus)国内外8个养殖群体进行遗传差异分析.结果表明:(1)8个群体的平均等位基因数(Na)为3.61～5.11,平均有效等位基因数(Ne)为2.81～3.76,平均观察杂合度(Ho)为0.861 1～0.925 0,平均期望杂合度(He)为0.603 9～0.697 7,平均多态信息含量(PIC)为0.522 9～0.637 3.表明这8个养殖群体具有丰富的遗传变异.(2)在对8个群体的遗传距离和遗传相似度进行估算基础上所作聚类分析表明, 8个群体分为两大支,3个"吉富"群体明显聚为一支,其他5个群体聚为一支, 反映了这8个群体的亲缘关系.(3)微卫星引物UNH187可以初步作为区分"新吉富"尼罗罗非鱼和其他尼罗罗非鱼群体的特异性标记.
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| 关 键 词： | 尼罗罗非鱼 微卫星 遗传差异 |
Genetic diversity in eight farmed Nile tilapia populations (Oreochromis niloticus) by microsatellite markers |
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| LI Xian-ren,LI Si-fa,TANG Shou-jie,CAI Wan-qi.Genetic diversity in eight farmed Nile tilapia populations (Oreochromis niloticus) by microsatellite markers[J].Journal of Shanghai Fisheries University,2009,18(1). |
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| Authors: | LI Xian-ren LI Si-fa TANG Shou-jie CAI Wan-qi |
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| Institution: | LI Xian-ren,LI Si-fa,TANG Shou-jie,CAI Wan-qi(The Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecology Certificated by the Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | To access Nile tilapia's genetic diversity,158 samples of eight farmed Nile tilapia populations were amplified by eighteen pairs of microsatellite primers.The result shows that:(1) In all eight populations the value of average allele number(Na) was 3.61-5.11,the value of average of effective number of alleles(Ne) was 2.81-3.76;the value of average observed heterozygosity(Ho) was 0.861 1-0.925 0,the value of average expected heterozygosity(He) was 0.603 9-0.697 7 and the value of average polymorphism informa... |
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| Keywords: | Oreochromis niloticus microsatellite genetic diversity |
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