| 不同激素配比对百合鳞片愈伤组织诱导的影响 |
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| 引用本文： | 崔瑞峰,张丽霞,韩晓红.不同激素配比对百合鳞片愈伤组织诱导的影响[J].黑龙江农业科学,2014(9):8-9. |
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| 作者姓名： | 崔瑞峰 张丽霞 韩晓红 |
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| 作者单位： | 1. 安阳工学院生物与食品工程学院,河南安阳,455000
2. 郑州大学教育系,河南郑州,450006 |
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| 摘 要： | 为筛选出适宜百合鳞片愈伤组织诱导的最佳激素配比,以给百合大规模的培养与繁殖创造有利的条件,以百合鳞片作为外植体,利用正交试验设计,研究不同激素配比对百合鳞片愈伤组织诱导的影响。结果表明:以MS为基本培养基,2,4-D浓度,6-BA浓度,NAA浓度3个因素对愈伤组织诱导率的影响表现为6-BANAA2,4-D。最优的激素配比组合为A2B1C3,即2,4-D 0.6 mg·L-1,6-BA 0.3 mg·L-1,NAA0.9mg·L-1,其愈伤组织诱导率为91.21%。
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| 关 键 词： | 百合 鳞片 愈伤组织 诱导 |
Effect of Different Hormone Combinations on Callus Induction Frequency of Lily Scale |
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| CUI Rui-feng,ZHANG Li-xia,HAN Xiao-hong.Effect of Different Hormone Combinations on Callus Induction Frequency of Lily Scale[J].Heilongjiang Agricultural Science,2014(9):8-9. |
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| Authors: | CUI Rui-feng ZHANG Li-xia HAN Xiao-hong |
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| Institution: | CUI Rui-feng, ZHANG Li-xia, HAN Xiao-hong (1. College of Biology and Food Engineering, Anyang Institute of Technology, Anyang, Henan 455000 2. Education Department of Zhengzhou University, Zhengzhou, Henan 450006) |
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| Abstract: | For screening suitable optimized hormone combinations for inducing callus of lily scale ,so as to create advantage conditions for culturing and propagating on a large scale ,taking lily scales as explants ,the effect of different hormone combinations on callus induction frequency of lily scales were studied with orthogonal de-sign .The results showed that ：taking MS as the basic culture medium ,the effect of hormones on callus induc-tion frequency was 6-BA〉NAA〉2 ,4-D .The optimized hormone combinations for inducing callus of lily scale was 2 ,4-D 0 .6 mg·L-1 ＋6-BA0 .3 mg·L-1 ＋NAA 0 .9 mg＆#183;L-1 ,the callus induction frequency was 91 .21% . |
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| Keywords: | L ilium bulbi f erum scale callus induction |
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