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甘肃野生草地早熟禾萌发期抗旱性鉴定与评价
引用本文:牛奎举,金小煜,李慧萍,徐海鹏,金宁,马晖玲.甘肃野生草地早熟禾萌发期抗旱性鉴定与评价[J].草地学报,2016,24(5):1041-1049.
作者姓名:牛奎举  金小煜  李慧萍  徐海鹏  金宁  马晖玲
作者单位:甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州,730070;甘肃农业大学园艺学院,甘肃兰州,730070
基金项目:国家自然科学基金(31160482)
摘    要:采用-0.1,-0.3,-0.5和-0.7 MPa的聚乙二醇6000(PEG-6000)模拟不同程度的干旱胁迫,在萌发期分别处理8份野生草地早熟禾(Poa pratensis)种质和2个商用品种21d,并测定发芽率、发芽势和活力指数等7个鉴定指标,并以其相对值作为评价指标,利用隶属函数法和聚类分析对供试的10份种质材料进行了综合评价和分类,同时,对各鉴定指标在草地早熟禾萌发期抗旱性评价中的作用进行了比较分析。结果表明,用于草地早熟禾萌发期抗旱性研究的适宜PEG-6000浓度为-0.1~-0.3 MPa,种子活力指数和发芽率受干旱胁迫的影响较大,且与抗旱性的关系最为密切,可作为草地早熟禾种质萌发期抗旱性鉴定的重要指标。10份种质材料抗旱性由强到弱依次为:秦州西和陇西安定渭源天祝榆中MidnightⅡ清水Baron;聚类分析可把供试材料按抗旱性强弱分为三类,其中清水和Baron属于干旱敏感种质;安定、渭源和MidnightⅡ等属于中等抗旱种质;秦州、西和和陇西属于抗旱种质,可作为抗旱育种材料。

关 键 词:野生草地早熟禾  萌发期  抗旱性  隶属函数  聚类分析

Identification and Evaluation of Wild Poa pratensis Varieties from Gansu for Drought Resistance During Germination Stage
NIU Kui-ju,JIN Xiao-yu,LI Hui-ping,XU Hai-peng,JIN Ning,MA Hui-ling.Identification and Evaluation of Wild Poa pratensis Varieties from Gansu for Drought Resistance During Germination Stage[J].Acta Agrestia Sinica,2016,24(5):1041-1049.
Authors:NIU Kui-ju  JIN Xiao-yu  LI Hui-ping  XU Hai-peng  JIN Ning  MA Hui-ling
Abstract:Different concentrations of PEG-6000 (-0.1,-0.3,-0.5 and-0.7 MPa) was used to simulate drought stress environment.Germination rate,germination potential,vigor index and other four identification indices of 8 wild Poa pratensis varieties and 2 cultivars were measured under drought stress during germination stage.According to the relative value of seven identification indices as evaluation indices,drought resistance of Poa pratensis varieties was comprehensively evaluated and classified by the methods of membership function and cluster analysis.Furthermore,the role of each identification indices in evaluating drought resistance of different varieties was compared.The results showed that-0.1~-0.3MPa PEG-6000 was the optimum osmotic stress concentration to study drought resistance of Poa pratensis at germination stage.Seed vigor index and germination rate were more susceptible to drought resistance and closer to the comprehensive evaluation value of drought resistance,and it could be used as the most important indices for identification of drought resistance of Poa pratensis.The drought resistance of all varieties decreased in the order as:Qinzhou,Xihe,Longxi,Anding,Weiyuan,Tianzhu,Yuzhong,Midnight Ⅱ,Qingshui and Baron.The cluster analysis showed that Qingshui and Baron were sensitive to drought stress;three varieties had medium drought tolerance,for example Anding,Weyuan and Midnight Ⅱ;Qinzhou,Xihe and Longxi were the highest drought tolerant,which can be used to breeding new drought tolerant cultivars in the future.
Keywords:Wild Poa pratensis  Germination stage  Drought resistance  Membership function  Cluster analysis
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