| 章姬草莓花药组织培养脱毒快速繁殖技术的研究 |
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| 引用本文： | 肖君泽,黄益鸿,姜放军,邓沛怡,阎珂.章姬草莓花药组织培养脱毒快速繁殖技术的研究[J].江西农业学报,2011(10):49-50,54. |
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| 作者姓名： | 肖君泽 黄益鸿 姜放军 邓沛怡 阎珂 |
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| 作者单位： | 湖南生物机电职业技术学院; |
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| 基金项目： | 湖南省农业厅重点科研课题“章姬草莓脱毒快繁技术研究”(20097614) |
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| 摘 要： | 研究了采用花药组织培养法快速繁殖章姬草莓脱毒苗的技术。结果表明:外植体宜选择处于单核期的花粉(其花蕾直径在4 mm左右,花瓣尚未开裂,花药直径约1 mm且呈淡黄色);诱导愈伤组织形成和分化不定芽的最佳培养基均为MS+6-BA 2.0 mg/L;最佳增殖培养基为MS+6-BA 1.5 mg/L。
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| 关 键 词： | 章姬草莓 花药 组织培养 脱毒 快速繁殖 |
Study on Virus-free Rapid Propagation of Zhangji Strawberry by Anther Tissue Culture |
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| XIAO Jun-ze,HUANG Yi-hong,JIANG Fang-jun,DENG Pei-yi,YAN Ke.Study on Virus-free Rapid Propagation of Zhangji Strawberry by Anther Tissue Culture[J].Acta Agriculturae Jiangxi,2011(10):49-50,54. |
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| Authors: | XIAO Jun-ze HUANG Yi-hong JIANG Fang-jun DENG Pei-yi YAN Ke |
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| Institution: | XIAO Jun-ze,HUANG Yi-hong,JIANG Fang-jun,DENG Pei-yi,YAN Ke(Hunan Biological and Electromechanical Polytechnic,Changsha 410127,China) |
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| Abstract: | The virus-free rapid propagation technique of Zhangji strawberry was studied by using anther tissue culture method.The results showed that it was suitable to choose the uninucleate pollens as the explants(the diameter of the alabastrum was about 4 mm,the petal had not crazed yet,the diameter of the anther was about 1 mm and it appeared yellow).The optimum medium for both callus formation inducement and adventitious bud differentiation was MS+6-BA 2.0 mg/L,and the optimum medium for rapid propagation was MS+... |
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| Keywords: | Zhangji strawberry Anther Tissue culture Virus-free Rapid propagation |
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