| 1株鸭源新城疫病毒的分离鉴定及生物学特性研究 |
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| 引用本文： | 刘大伟,焦培荣,黎先伟,何琴,高诗敏,漆丹丹,陈德成,任涛.1株鸭源新城疫病毒的分离鉴定及生物学特性研究[J].中国畜牧兽医,2011,38(5):143-145. |
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| 作者姓名： | 刘大伟 焦培荣 黎先伟 何琴 高诗敏 漆丹丹 陈德成 任涛 |
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| 作者单位： | 华南农业大学兽医学院,农业部动物疫病防控重点开放实验室, 广东省动物源性人兽共患病预防与控制重点实验室,广东广州 510642 |
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| 基金项目： | 国家自然科学基金，公益性行业(农业)科研专项经费 |
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| 摘 要： | 从广东地区发病鸭中分离到1株新城疫病毒,血凝试验、血凝抑制试验结果表明此分离株具有血凝活性,且这种血凝性可被NDV标准阳性血清抑制,而不能被AIV-H5、AIV-H7、AIV-H9、EDSV阳性血清抑制;用针对NDV F基因特异性鉴定引物对该分离株进行聚合酶链反应扩增,可扩增出相应的目的片段。由此,初步鉴定该分离株为鸭源新城疫病毒,并命名为NDV/DUCK/SD/2006。另外,通过毒力测定,该毒株的MDT、IVPI分别为51.75 h、2.44,判定为强毒力株。
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| 关 键 词： | 新城疫病毒 鸭 广东 生物学特性 |
| 收稿时间： | 2010-10-27 |
Identificationand Biological Characterization of a Newcastle Disease Virus from Duck |
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| LIU Da-wei,JIAO Pei-rong,LI Xian-wei,HE Qin,GAO Shi-min,QI Dan-dan,CHEN De-cheng,REN Tao.Identificationand Biological Characterization of a Newcastle Disease Virus from Duck[J].China Animal Husbandry & Veterinary Medicine,2011,38(5):143-145. |
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| Authors: | LIU Da-wei JIAO Pei-rong LI Xian-wei HE Qin GAO Shi-min QI Dan-dan CHEN De-cheng REN Tao |
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| Institution: | Key Lab of Zoonoses Control and Prevention of Guangdong,College of Veterinary Medicine,Key Lab of Animal Disease Control and Prevention of Ministry of Agriculture,South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | A Newcastle disease virus(NDV) was isolated from duck in Guangdong. It was identified with hemagglutination,hemagglutination inhibition. These results showed the isolate had hemagglutination and was inhibited by the standard antibody of AIV-H5,AIV-H7,AIV-H9 and EDSV. It was amplified by RT-PCR and sequenced,which was used by the specific identification primers of NDV F gene. Thus,the isolate was identified as a duck NDV,named NDV/DUCK/SD/2006. In this research,the isolate were conducted virulence determination. Results showed that,the MDT,and IVPI of the isolate were 51.75 h,2.44 respectively,suggesting it was a velogenic strain. |
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| Keywords: | NDV duck Guangdong biological characterization |
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