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盘羊刚果嗜皮菌的分离与鉴定
引用本文:丁国婵,何传雨,王静梅,剡根强.盘羊刚果嗜皮菌的分离与鉴定[J].中国畜牧兽医,2011,38(4):191-193.
作者姓名:丁国婵  何传雨  王静梅  剡根强
作者单位:石河子大学动物科技学院,新疆石河子 832003
摘    要:从疑似刚果嗜皮菌感染的盘羊皮肤病料中分离刚果嗜皮菌,并进行鉴定。将皮肤病料于28 ℃风干、粉碎,加无菌水浸泡15 d,静置,取上清加6%酵母膏液,60 ℃条件下30 min,混匀后连续10倍倍比稀释涂板,37 ℃培养48 h后挑取可疑菌落进行细菌形态学、培养特性、间接免疫酶标染色法鉴定,并根据GenBank上发表的刚果嗜皮菌16S rRNA序列设计特异性引物对分离株进行PCR扩增,得到与预期相符的606 bp基因片段,结果证实该盘羊分离株为刚果嗜皮菌。

关 键 词:刚果嗜皮菌  分离鉴定  间接免疫酶标染色法  16S  rRNA  
收稿时间:2010-10-25

Isolation and Identification of Dermatophilus congolensis of Argali Sheep
DING Guo-chan,HE Chuan-yu,WANG Jing-mei,YAN Gen-qiang.Isolation and Identification of Dermatophilus congolensis of Argali Sheep[J].China Animal Husbandry & Veterinary Medicine,2011,38(4):191-193.
Authors:DING Guo-chan  HE Chuan-yu  WANG Jing-mei  YAN Gen-qiang
Institution:College of Animal Science and Technology,Shihezi University,Shihezi 832003,China
Abstract:To isolate and identificate Dermatophilus congolensis from Argali sheep,the skin material was dried at 28 ℃ and smashed,added with sterile water for 15 days,standing. Added with 6% yeast extract,stirred at 60 ℃ for 30 min,scribbled on plates and placed at 37 ℃ for 48 h to evaluate the characteristics of the Dermatophilus congolensis stains,the bacterial morphology,cultivating characteristics,indirect immunohistochemistry were performed and devised a pair of specific primers according to the 16S rRNA sequence of Dermatophilus congolensis GenBank published for PCR.The results showed the bacteria collected from Argali sheep was related to the Dermatophilus congolensis.
Keywords:Dermatophilus congolcnsis  isolation and identification  indirect immunohistochemistry  16S rRNA
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