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Bt杀虫蛋白对棉铃虫的抗虫毒力和增效作用
引用本文:范贤林,赵建周,芮昌辉,谢天健.Bt杀虫蛋白对棉铃虫的抗虫毒力和增效作用[J].农药学学报,1999,1(3):51-55.
作者姓名:范贤林  赵建周  芮昌辉  谢天健
作者单位:1.中国农业科学院植物保护研究所,北京100094
基金项目:国家自然科学基金! (No.3950 0 10 0 )资助课题
摘    要:用 Bt杀虫蛋白 (ICP) Cry1A汰选的棉铃虫 (H elicoverpa armigera)抗性种群和同源对照种群分别测定了 4种不同类型 Bt ICP的抗虫毒力。结果表明 :4种杀虫蛋白对对照种群的毒力顺序为 :Cry1Ac>Cry1Ac+ 1C>Cry2 A>Cry1C;对抗性种群的毒力顺序为 :Cry1Ac+ 1C>Cry1Ac>Cry2 A>Cry1C,其中Cry1Ac+ 1C表现出对抗性种群有显著的协同增效作用。采用从 Bt液体发酵上清液中提取的增效粉 ,与Cry1Ac以 1∶ 1和 1∶ 2的比例混合 ,对 Cry1Ac有显著的增效作用 ,用抗性种群测定的增效比值明显大于对照种群 ,处理 5d的增效比值大于 14d的

关 键 词:苏云金杆菌    杀虫蛋白    棉铃虫    抗虫毒力    增效作用

SYNERGISM AND TOXICITY OF INSECTICIDAL PROTEINS FROM BACILLUS THURINGIENSIS TO THE COTTON BOLLWORM
Fan Xian-lin,Zhao Jian-zhou,Rui Chang-hui and Xie Tian-jian.SYNERGISM AND TOXICITY OF INSECTICIDAL PROTEINS FROM BACILLUS THURINGIENSIS TO THE COTTON BOLLWORM[J].Chinese Journal of Pesticide Science,1999,1(3):51-55.
Authors:Fan Xian-lin  Zhao Jian-zhou  Rui Chang-hui and Xie Tian-jian
Institution:1.Institute of Plant Protection2.Chinese Academy of Agricultural Sciences
Abstract:Toxicity of 4 kinds of insecticidal crystal protein (ICP) from Bacillus thuringiensis (Bt) to Cry1Ac-resistant (R) and the homologous susceptible (S) strains of cotton bollworm, Helicoverpa armigera Hübner was evaluated. The sequence of toxicity of the 4 Bt ICP is as follows: Cry1Ac>Cry1Ac+1C>Cry2A>Cry1C to S strain, and Cry1Ac+1C>Cry1Ac>Cry2A>Cry1C to R strain. Significant synergism was found while appling Cry1c and Cry1c together to the R strain. At 1∶1 and 1∶2 proportions of the synergist and Cry1Ac notable synergism was observed. The synergism after 5 days treatment was higher than that after 14 days treatment, and was higher in the R strain than in the S strain.
Keywords:Bacillus thuringiensis  Helicoverpa armigera  Toxicity  Synergism
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