| 耐草甘膦菜豆耐性机理的初步研究 |
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| 引用本文： | 向文胜,赵长山,陶波,王相晶,苏少泉,张文吉.耐草甘膦菜豆耐性机理的初步研究[J].农药学学报,1999,1(3):33-38. |
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| 作者姓名： | 向文胜 赵长山 陶波 王相晶 苏少泉 张文吉 |
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| 作者单位： | 1.东北农业大学生命科学与生物技术研究中心,哈尔滨150030 |
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| 基金项目： | 黑龙江省科委青年计划项目，国家自然科学基金! (396 70 4 98)资助项目 |
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| 摘 要： | 采用液谱测定耐性、感性菜豆叶片对草甘膦的吸收及草甘膦传导入根中的量。耐性、感性菜豆吸收、传导草甘膦无差异。耐性、感性菜豆 EPSP合成酶提取物中的蛋白质含量分别为 3.0 0 mg/ m L和 3.0 8mg/ m L ,EPSP合成酶的比活性分别为 2 .13nmol· min-1· mg-1蛋白和 1.97nmol· min-1· mg-1蛋白 ,但耐性、感性菜豆 EPSP合成酶比活性被草甘膦不同浓度抑制的差异大 ,抑制耐性菜豆 EPSP合成酶活性的草甘膦浓度 I50 为 19.2μmol/ L ,而感性的 I50 为 6 .3μmol/ L。两种菜豆对草甘膦的耐性差异在于各自的 EPSP合成酶比活性被草甘膦的抑制程度不同。
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| 关 键 词： | 菜豆 草甘膦 耐性机理 EPSP合成酶 |
PRELIMINARY STUDIES ON THE MECHANISM OF GLYPHOSATE TOLERANCE IN BEAN |
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| Xiang Wen-sheng,Zhao Chang-shan,Tao Bo,Wang Xiang-jing,Su Shao-quan and Zhang Wen-ji.PRELIMINARY STUDIES ON THE MECHANISM OF GLYPHOSATE TOLERANCE IN BEAN[J].Chinese Journal of Pesticide Science,1999,1(3):33-38. |
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| Authors: | Xiang Wen-sheng Zhao Chang-shan Tao Bo Wang Xiang-jing Su Shao-quan and Zhang Wen-ji |
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| Institution: | 1.Life Science & Biotechnique Center2.Northeast Agriculture University |
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| Abstract: | Foliar absorption of glyphosate and the quantity translocated into root of tolerant and susceptible bean were determined by Liquid Chromatographic method, but no difference was found. After 72h and 120h of foliar treatment there was also no difference in glyphosate translocation. Protein content of EPSP synthase in extracts from tolerant and susceptible bean are 3.00 mg/mL and 3.08 mg/mL, respectively. Specific activity of EPSP synthase are 2.13 nmol·min -1·mg -1 protein and 1.97 nmol·min -1·mg -1 protein, respectively. But glyphosate inhibited EPSP synthase activity in tolerant and susceptible biotypes uneaqually. EPSP synthase activity of tolerant and susceptible biotypes was inhibited approximately 50% by 19.2 μmol/L and 6.3 μmol/L glyphosate, respectively. The current research has shown that difference between beans in glyphosate tolerance consist in the difference of EPSP synthase activity by glyphosate inhibition. |
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| Keywords: | Bean Glyphosate Tolerant mechanism EPSP synthase |
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