| 一种固定化酶对氰戊菊酯的降解特性 |
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| 引用本文： | 虞云龙,史锋,樊德方,盛国英,傅家谟.一种固定化酶对氰戊菊酯的降解特性[J].农药学学报,1999,1(1):74-77. |
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| 作者姓名： | 虞云龙 史锋 樊德方 盛国英 傅家谟 |
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| 作者单位： | 1.浙江大学植保系,杭州310029 |
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| 基金项目： | 国家自然科学基金 !(39870 50 4、2 94 772 75)，浙江省教委资助项目 |
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| 摘 要： | 用海藻酸钙凝胶将 Alcaligenes sp.YF11菌提取的降解酶制成固定化酶。测定了固定化酶对氰戊菊酯的降解特性 ,测得最适 p H为 8.0 ,最适温度为 35℃ ,Km为 55.0 nmol/ml。 2 5℃时 ,流速为 4 ml/min,固定化酶柱对 3.1- 2 0 0 mg/L的氰戊菊酯的去除率为 6 1.3% - 90 %
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| 关 键 词： | 农药 降解 酶 |
DEGRADATION CHARACTERISTICS OF AN IMMOBILIZED ENZYME FOR FENVALERATE |
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| Yu Yun-long,Shi Feng,Fan De-fang,Sheng Guo-yin and Fu Jia-mo.DEGRADATION CHARACTERISTICS OF AN IMMOBILIZED ENZYME FOR FENVALERATE[J].Chinese Journal of Pesticide Science,1999,1(1):74-77. |
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| Authors: | Yu Yun-long Shi Feng Fan De-fang Sheng Guo-yin and Fu Jia-mo |
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| Institution: | 1.Department of Plant Protection2.Zhejiang University |
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| Abstract: | The cell free preparation, extracted from Alcaligenes sp. YF11 capable of degrading the target pesticides, was immobilized with Ca alginate. Degradative nature of the immobilized enzyme was then determined with fenvalerate as substrate. The optimal occurred at pH8.0 and temperature of 35℃. Km for degradation of fenvalerate was measured to be 55.0nmol/ml. An immobilized enzyme column was examined for its use in detoxification of fenvalerate at flow rate of 4ml/min under temperature of 25℃, 61.3% 90% of fenvalerate were removed at concentrations of 3.1 200mg/ml. |
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| Keywords: | pesticide degradation enzyme |
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