| 影响猪生长激素基因工程菌表达效率的因素研究 |
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| 引用本文： | 尹德钟,张海峰,贾锋,齐顺章.影响猪生长激素基因工程菌表达效率的因素研究[J].中国农业大学学报,1993(1):35-38. |
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| 作者姓名： | 尹德钟 张海峰 贾锋 齐顺章 |
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| 作者单位： | 北京农业大学生物学院; |
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| 摘 要： | 在摇瓶条件下研究了影响猪生长激素(pGH)基因工程菌表达效率的某些因素。实验结果表明:工程菌 pJW301/JA221在不同光密度时开始诱导,诱导不同时间及不同通气量对pGH 的表达效率影响较大;过夜培养物存放时间的长短及不同氨苄青霉素(Amp)浓度对表达效率稍有影响。表达载体 pJW301转化不同受体菌,其表达效率有较大差异。
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| 关 键 词： | 猪生长激素 表达效率 表达载体 |
| 收稿时间： | 1992/3/17 0:00:00 |
Investigation of Impacts of Some Factors Affecting the Expression of Porcine Growth Hormone Gene in E.coli |
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| Yin Dezhong Zhang Haifeng Jia Feng Qi Shunzhang.Investigation of Impacts of Some Factors Affecting the Expression of Porcine Growth Hormone Gene in E.coli[J].Journal of China Agricultural University,1993(1):35-38. |
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| Authors: | Yin Dezhong Zhang Haifeng Jia Feng Qi Shunzhang |
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| Institution: | Yin Dezhong Zhang Haifeng Jia Feng Qi Shunzhang (College of Biology) |
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| Abstract: | We have studied the impacts of some factors on the expression of porcine growth hormone (pGH)gene in E.coli.The results showed that the production of the pGH in genetically engineered bacterium pJW301/JA221 was greatly affected by bacterial deasity at the beginning of induction,in- ducing duration and areation value;and the storing period of overnight culture and the concentration of ampicillin had only a little impact on the pGH production.The expression rate of pGH gene also varied in different host strai... |
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| Keywords: | porcine growth hormone expression rate expression vector |
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