Genetic diversity and phylogenic relationships in date-palms (Phoenix dactylifera L.) as assessed by random amplified microsatellite polymorphism markers (RAMPOs) |
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| Authors: | Soumaya Rhouma Sonia Dakhlaoui-Dkhil Ali Ould Mohamed Salem Salwa Zehdi-Azouzi Abdelmajid Rhouma Mohamed Marrakchi Mokhtar Trifi |
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| Institution: | 1. Laboratory of Molecular Genetics, Immunology & Biotechnology, Faculty of Sciences of Tunis, Campus University, 2092 El Manar Tunis, Tunisia;2. Department of Biologie, Faculty of Sciences & Techniques, University of Nouakchott, B.P. 5026, Mauritania;3. INRAT, CRRAO, 2260 Degache, Tunisia |
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| Abstract: | Random amplified microsatellite polymorphisms (RAMPOs) were used to assess genetic diversity among 30 date-palm cultivars and 10 male trees. Using 18 primers combinations, 197 bands were scored and 186 were polymorphic suggesting the high level of polymorphism among studied cultivars. Moreover, taking into account the high percentage of polymorphic bands (ppb), the resolving power (Rp) together with the polymorphism information content (PIC) scored values, all the tested primer sets contribute strongly in the discrimination of date-palm genotypes. In addition, the topology of the derived UPGMA dendrogram exhibited cultivars’ clustering made independently both from the geographical origin and/or from the sex of trees. The present data support the Mesopotamian origin of the date-palm domestication. Thus we assume that the used method is efficient to assess genetic diversity within date-palm cultivars. Data are discussed in relation with the opportunity of the RAMPO method to provide additional molecular markers suitable in the improvement of the date-palms germplasm characterisation. |
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| Keywords: | Phoenix dactylifera RAMPO Polymorphism Relationships |
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