Molecular cloning and characterization of a cucumber MAP kinase gene in response to excess NO3 and other abiotic stresses |
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| Authors: | Huini Xu Xiufeng Wang Xudong Sun Qinghua Shi Fengjuan Yang Dongliang Du |
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| Institution: | 1. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, PR China;2. State Key Laboratory of Crop Biology, Tai’an 271018, PR China |
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| Abstract: | Mitogen-activated protein kinases (MAPKs) play important roles in the transduction of extracellular signals to the intracellular targets in all eukaryotes. Here, a cucumber cDNA designated CsNMAPK, encoding a mitogen-activated protein kinase was isolated using RT-PCR, 3′ and 5′ RACE. The full-length cDNA sequence contains 1636 bp and an open reading frame (ORF) of 1113 bp, which encodes 370 amino acid residues. According to the phylogenetic analysis, CsNMAPK belongs to subgroup I MAPK in plants. Northern blot analysis revealed that CsNMAPK expressed differently in response to excess NO3−. And the CsNMAPK expression kinetics between a salt-resistant cultivar (Xintaimici) and a salt-sensitive cultivar (Shennongchunwu) was slightly different under 182 mmol L−1 NO3− treatment. The mRNA levels also increased after 24 h treatments with H2O2 and salicylic acid (SA), but decreased with abscisic acid (ABA) and low-temperature. However, there was no significant induction of CsNMAPK gene after 24 h drought and high-temperature treatments. Our results suggested that a MAP kinase cascade may function in excess NO3− and other abiotic stresses in cucumber. |
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| Keywords: | Mitogen-activated protein kinase Cucumber Excess NO3&minus Abiotic stresses |
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