| 杉木优树分子遗传变异的研究 |
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| 引用本文： | 李梅,施季森,何祯祥,易能君.杉木优树分子遗传变异的研究[J].林业科学,2001,37(4):137-141. |
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| 作者姓名： | 李梅 施季森 何祯祥 易能君 |
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| 作者单位： | 南京林业大学林木遗传和基因工程实验室 |
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| 基金项目： | 国家自然科学基金的资助 (资助号 3 9470 5 91) |
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| 摘 要： | 种质资源的收集和管理是一项复杂而艰巨的工作 (Ｖｉｒｋｅｔａｌ.,1 995) ,其遗传变异研究对林木遗传改良具有重要意义 (Ｌｉｕｅｔａｌ.,1 993)。遗传变异研究既可以用谱系或杂种的表型资料 ,也可以用同工酶数据 ,还可用ＤＮＡ水平的分子标记数据 (Ｓｍｉｔｈｅｔａｌ.,1 992 )。近年来 ,分子标记已广泛而有效地用于估测育种材料的遗传变异 (Ｌｅｅ ,1 995) ,其中ＲＡＰＤ(ＲａｎｄｏｍａｍｐｌｉｆｉｅｄｐｏｌｙｍｏｒｐｈｉｃＤＮＡ ,随机扩增多态性ＤＮＡ)是较好的一种分子标记技术 (Ｗｅｌｓｈｅｔａｌ.,1 990 ;Ｔｉｎｋｅｒｅｔａｌ…
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| 关 键 词： | 杉木 优树 遗传变异 RAPD 遗传多态性 遗传相似性 遗传距离 聚类分析 |
| 修稿时间： | 1999年7月5日 |
STUDY ON MOLECULAR GENETIC VARIATION OF SUPERIOR TREES IN CHINESE FIR(CUNNINGHAMIA LANCEOLATA(LAMB.)HOOK. |
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| Li Mei\ Shi Jisen\ He Zhenxiang\ Yi Nengjun.STUDY ON MOLECULAR GENETIC VARIATION OF SUPERIOR TREES IN CHINESE FIR(CUNNINGHAMIA LANCEOLATA(LAMB.)HOOK.[J].Scientia Silvae Sinicae,2001,37(4):137-141. |
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| Authors: | Li Mei\ Shi Jisen\ He Zhenxiang\ Yi Nengjun |
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| Abstract: | Thirty superior trees of Chinese fir from eight provinces were analyzed on genetic variation with RAPD method.A total of 284 bands,including 190 polymorphic ones,were produced with 26 primers, and mean genetic distance between the thirty superior trees was 0.4909,indicating a high level of genetic diversity among the tress tested.Two superior trees,coded as ZJZ 01 and JXS26,showed a high value in genetic similarity,which meant that only one of them could be essentially preserved in gene bank.The clustering analysis indicated that most of the superior trees could be clustered at a relatively high genetic distance and rich genetic variation could be expected in the superior tree population.This might be valuable for marker-assisted germplasm collection,parent grouping in hybridization and genetic diversity evaluation on breeding materials in improvement of Chinese fir. |
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| Keywords: | Chinese fir Superior trees Genetic variation RAPD |
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