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| 牛乳中三种雌激素残留的HPLC检测法 | |
| 引用本文： | 赵金莲,杜兰祥,李月娟,许敬东.牛乳中三种雌激素残留的HPLC检测法[J].甘肃农业大学学报,2009,44(2). |
| 作者姓名： | 赵金莲 杜兰祥 李月娟 许敬东 |
| 作者单位： | 1. 甘肃农业大学,甘肃,兰州,730070 2. 兰州市安宁区疾控中心,甘肃,兰州,730070 3. 甘肃省产品质量监督检验中心,甘肃,兰州,730000 |
| 摘 要： | 建立了牛奶中3种雌激素残留量(雌二醇、己烯雌酚、雌酮)的HPLC(高效液相色谱)检测方法.得到色谱条件为流动相:乙腈-水(体积比为1∶1);检测波长:230 nm;流速1.0 mL.min-1.采用该方法测定了市售鲜牛奶中3种雌激素的残留量,结果表明:其分离效果良好,平均回收率范围82.2%~95.5%,相对标准偏差为2.0%~4.7%,最低检出限达1.0~2.0 ng.mL-1.该方法具有灵敏、准确、精密、无杂质干扰等优点. |
| 关 键 词： | 牛奶 雌激素 高效液相色谱法 |
Determination of estrogen residues in milk by HPLC |
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| ZHAO Jin-lian,DU Lan-xiang,LI Yue-juan,XU Jing-dong.Determination of estrogen residues in milk by HPLC[J].Journal of Gansu Agricultural University,2009,44(2). | |
| Authors: | ZHAO Jin-lian DU Lan-xiang LI Yue-juan XU Jing-dong |
| Institution: | ZHAO Jin-lian1,DU Lan-xiang2,LI Yue-juan3,XU Jing-dong(1.Gansu Agricultural University,Lanzhou 730070,China,2.The Center of Control of Disease in Anning District,3.The Center of Inspection and Monitoring of Production Quality in Gansu Province,Lanzhou 730000,China) |
| Abstract: | To study an efficient method for dertermination of three estrogens(estradiol,diethylstilbestrol and norethisterone) in milk,the HPLC was developed by using acetonitrile,the supernatant from the samples with the protein precipitated and removed was concentrated using HPLC,acetonitrile-water(volume ratio of 1∶1) as mobile phase for analysis.The results indicated that good separation was achieved in 3 components and the recovery rate was between 82.2 % and 95.5 %,the relative standard deviation was between 2.0 % and 4.7 %,the lowest detection limit was between 1.0 ng·mL-1 and 2.0 ng·mL-1.The method was proved to be satisfactory in precision,acuracy and sensitivity. |
| Keywords: | milk estrogen high performance liquid chromatography |
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