| 大豆育种过程中亲子遗传关系的RAPD研究初报 |
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| 引用本文： | 陈绍江,惠东威.大豆育种过程中亲子遗传关系的RAPD研究初报[J].东北农业大学学报,1996,27(2):137-141. |
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| 作者姓名： | 陈绍江 惠东威 |
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| 作者单位： | 东北农业大学大豆所，中国科学院遗传所，河南农科院经作所，中国农业大学植物遗传育种系 |
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| 摘 要： | 试验以组合８００２４×中１９亲本及其育成品系Ｂ_１，Ｂ_２为材料，用ＲＡＰＤ技术对其亲子关系进行了研究．１０个随机引物（１０ｂｐｓｅ）的ＰＣＲ扩增结果表明，双亲在遗传上有较大差异，母本８００２４有１３条特征带，父本中１９有６条特征带．其在子代中能够重组，使Ｂ_１，Ｂ_２均具有双亲ＲＡＰＤ标记的特征．但经过多代选择，双亲ＲＡＰＤ特征在育成的子代品系中的分布是不均衡的．Ｂ_１更多地继承了母本８００２４的绝大部分ＲＡＰＤ特征，遗传上更近于母本．Ｂ_２则表现出倾向于父本．这一结果与田间观察结果基本一致，说明用ＲＡＰＤ追踪育种过程，探讨亲子遗传关系是可行的．另外，试验观察到子代品系在继承双亲ＲＡＰＤ特征的同时，还出现了新的子代所特有的ＲＡＰＤ特征．产生这种超亲ＲＡＰＤ特征的真实原因及其意义有待进一步研究．
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| 关 键 词： | RAPD，大豆，亲本关系，PCR |
A RAPD STUDY ON THE GENETIC RELATION SHIP BETWEEN PARENTS AND OFFSPRINGS IN SOYBEAN BREEDING |
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| Chen Shaojing,Yang Qingkai,Wang Jinling.A RAPD STUDY ON THE GENETIC RELATION SHIP BETWEEN PARENTS AND OFFSPRINGS IN SOYBEAN BREEDING[J].Journal of Northeast Agricultural University,1996,27(2):137-141. |
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| Authors: | Chen Shaojing Yang Qingkai Wang Jinling |
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| Abstract: | The parents 80024, Zhong 19 and their offsprings B_1and B_2 were used to analyse the genetic relationship between parents and offspring by RAPD.The PCR amplification results of 10 primers(10 bases pairs), showed an apparent genetic difference between the two parents, of which 80024 (the female) had 13 speeial bands and zhong 19(the male) had only 6.These special bands in parents were found to recombine in the offsprengs of B_1 and B_2, both of which had the RAPD characteristics of both parents. However the incidence of the bands was biased, B_1 was much morc close to 80024, while B_2 was somewhat close to Zhong 19. The results were in accordance with the observations from field. Theis suggested that RAPD markers were effective in detecting genetic relationships between parents and offsprings,providing a potential powerful tool for soybean breeding.Besides,a new,non-parenta band was found in both offsprings and the reason for this is unknown. |
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| Keywords: | RAPD soybean parent -offspring genetic relationgship PCR |
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