| 青稞“昆仑12号”HVA1基因的克隆与蛋白质结构预测 |
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| 引用本文： | 姚晓华,邓晓青,吴昆仑,张志斌.青稞“昆仑12号”HVA1基因的克隆与蛋白质结构预测[J].江西农业大学学报,2011(5):855-860. |
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| 作者姓名： | 姚晓华 邓晓青 吴昆仑 张志斌 |
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| 作者单位： | 青海省农林科学院/青稞遗传育种重点实验室/青海省高原作物种质资源创新与利用重点实验室; |
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| 基金项目： | 农业部948(引进国际先进农业科学技术)项目(2010-Z29); 青海大学中青年科研基金项目(2010-QN-04) |
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| 摘 要： | 抗旱性是青稞重要的耐逆性状.根据已报道的大麦抗旱相关基因HVA1的DNA序列,设计引物,以青稞品种昆仑12号经干旱处理8h处理后的幼苗为材料,进行PCR扩增,得到约700 bp的HVA1基因编码框的全序列,将其克隆到pMD20 -T上,重组子和酶切鉴定正确后,进行序列测定和分析.结果表明,与西藏强旱性青稞中HVA1基因...
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| 关 键 词： | 青稞 HVA1基因 基因克隆 序列分析 |
Cloning of HVA1 Gene and Predicting of Protein Structure in Hulless Barley Kunlun 12 |
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| YAO Xiao-hua,DENG Xiao-qing,WU Kun-lun,ZHANG Zhi-bin.Cloning of HVA1 Gene and Predicting of Protein Structure in Hulless Barley Kunlun 12[J].Acta Agriculturae Universitis Jiangxiensis,2011(5):855-860. |
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| Authors: | YAO Xiao-hua DENG Xiao-qing WU Kun-lun ZHANG Zhi-bin |
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| Institution: | YAO Xiao-hua,DENG Xiao-qing,WU Kun-lun*,ZHANG Zhi-bin(Qinghai Academy of Agricultural and Forestry Sciences,Xining 810016,China) |
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| Abstract: | Drought resistance is one of the most important characters of stress tolerance ability in hulless barley.Based on the DNA sequence of HVA1 gene in barley which is related to drought resistance,a pair of primers were designed.The specific fragments of about 700 bp in size were obtained,respectively,after PCR amplification using the seedlings cDNA of hulless barley variety Kunlun 12 under drought conditions after 8 h as template.Then they were cloned into vector pMD-20T,and sequenced after the identification ... |
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| Keywords: | hulless barley HVA1 gene gene cloning sequence analysis |
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