| 菌株N3和TP-3的分类鉴定及保水效果研究 |
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| 引用本文： | 黄海东,董瑞峰,班立桐,杨红澎,王玉,刘畅,王青.菌株N3和TP-3的分类鉴定及保水效果研究[J].天津农学院学报,2010,17(3):21-25. |
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| 作者姓名： | 黄海东 董瑞峰 班立桐 杨红澎 王玉 刘畅 王青 |
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| 作者单位： | 1. 天津农学院,农学系,天津,300384
2. 天津市花苗木服务中心,天津,300300 |
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| 基金项目： | 天津市高等学校科技发展基金计划项目"利用活性污泥合成中长链聚羟基脂肪酸酯的研究" |
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| 摘 要： | 用多相分类的方法对2株生物聚合物合成菌进行了鉴定,通过形态学、生理生化和16S rDNA系统进化分析,鉴定菌株N3为泛菌属,菌株TP3为鞘氨醇单胞菌属。N3和TP3合成的生物聚合物具有协效增粘性,将这两种菌剂混合喷洒在沙土表面,能够形成致密的结皮,从而显著提高沙土的持水能力;试验第15天,N3和TP3混合处理组的含水量为2.1%,是对照的10.5倍,是N3和TP3单独处理组含水量的3倍和1.6倍。这种由混合菌剂形成的结皮不会影响种子的萌发,且能增加幼苗的成活率。
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| 关 键 词： | 生物结皮 泛菌属 鞘氨醇单胞菌属 分类鉴定 |
Identification and Water Conservation Capacity of Strain N3 and TP-3 |
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| HUANG Hai-dong,DONG Rui-feng,BAN Li-tong,YANG Hong-peng,WANG Yv,LIU Chang,WANG Qing.Identification and Water Conservation Capacity of Strain N3 and TP-3[J].Journal of Tianjin Agricultural College,2010,17(3):21-25. |
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| Authors: | HUANG Hai-dong DONG Rui-feng BAN Li-tong YANG Hong-peng WANG Yv LIU Chang WANG Qing |
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| Institution: | 1.Department of Agronomy,Tianjin Agricultural University,Tianjin 300384,China;2.Tianjin Flower-plantlet Service Centre,Tianjin 300300,China) |
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| Abstract: | Strain N3 and TP3 synthesize biopolymers with properties of thickening.The taxonomic position was investigated by a polyphasic approach.According to the characteristics of morphology,physiology and biochemistry tests and phylogenetic analysis based on 16S rRNA gene sequences,strain N3 was identified as genus Pantoea,strain TP3 was identified as genus Sphingomonas.The biopolymers produced by strain N3 and TP-3 had definite synergistic thickening characteristics.The culturing solution of strain N3 and TP3 were sprinkled on the surface of sand,and the conglutinating sand with a compact bacteria crust could be formed and improve the water conservation capacity of the sand.The 15th day of the test,the water content of the experimental group treated simultaneously by N3 and TP3 reached 2.1%,10.5 times of the CK,and 3 and 1.6 times of the experimental group separately treated by N3 and TP3.This crust had not negative effects on seed germination,and coluld increase the survival rate of seedlings. |
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| Keywords: | biological crusts Pantoea sp Sphingomonas sp classification and identification |
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