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| 百合种质资源遗传多样性及亲缘关系的RAPD分析 | |
| 引用本文： | 童巧珍,周日宝,盛孝邦,刘湘丹,王朝晖,刘笑蓉,谭周进.百合种质资源遗传多样性及亲缘关系的RAPD分析[J].农业现代化研究,2009,30(4):509-512. |
| 作者姓名： | 童巧珍 周日宝 盛孝邦 刘湘丹 王朝晖 刘笑蓉 谭周进 |
| 作者单位： | 1. 湖南农业大学,湖南,长沙,410128;湖南中医药大学,湖南,长沙,410208 2. 湖南中医药大学,湖南,长沙,410208 3. 湖南农业大学,湖南,长沙,410128 |
| 基金项目： | 国家科技支撑项目，湖南省中医药管理局资助项目 |
| 摘 要： | 以百合新鲜鳞叶为材料,利用RAPD分子标记技术对16份不同生态型的百合种质的遗传多样性和亲缘关系进行了分析.研究结果表明,从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%.UPGMA聚类分析结果表明.16份百合种质在阀值为0.9407处分为2个聚类群,即百合科两个不同属的植物:百合属和大百合属;阈值为0.5790处,百合属被分为3个不同的种:百合、卷丹和细叶百合.聚类结果和亲缘关系分析表明不同供试百合之间的遗传多样性较高,亲缘关系较远,且各种质遗传距离与地理距离具有一定的相关性. |
| 关 键 词： | 百合 种质资源 聚类分析 |
Genetic Diversity and Phylogenetic Relationship Analysis on Germplasm Resources of Lily by RAPD |
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| TONG Qiao-zhen,ZHOU Ri-bao,SHENG Xiao-bang,LIU Xiang-dan,WANG Zhao-hui,LIU Xiao-rong and TAN Zhou-jin.Genetic Diversity and Phylogenetic Relationship Analysis on Germplasm Resources of Lily by RAPD[J].Research of Agricultural Modernization,2009,30(4):509-512. | |
| Authors: | TONG Qiao-zhen ZHOU Ri-bao SHENG Xiao-bang LIU Xiang-dan WANG Zhao-hui LIU Xiao-rong and TAN Zhou-jin |
| Institution: | 1.Hunan Agricultural University;Changsha;Hunan 410128;China;2.Hunan University of Chinese Medicine;Hunan 410208;China |
| Abstract: | In this study,the genetic diversity and phylogenetic relationship among 16 genotypes of Lily collected from different ecological sources was analyzed by RAPD. Thirty-five available RAPD primers were screened from 120 random primers,and a total of 769 DNA bands were amplified,in which 628 bands were polymorphic,the percentage is 81.7%. Cluster analysis showed that the 16 Lily germplasms were divided into two groups at 0.9407. Corresponding to two genus in liliaceae:Lilium and Cardiocrinum,and that germplasms... |
| Keywords: | RAPD lily germplasm resources RAPD cluster analysis |
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