| 大豆抗旱种质资源遗传多样性的SSR分析 |
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| 引用本文： | 王振东,陈超力,于佰双,李进荣,王惠.大豆抗旱种质资源遗传多样性的SSR分析[J].大豆科学,2010,29(3). |
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| 作者姓名： | 王振东 陈超力 于佰双 李进荣 王惠 |
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| 作者单位： | 沈阳农业大学,生物科学技术学院,辽宁,沈阳,110866;黑龙江省农业科学院,大豆研究所,黑龙江,哈尔滨,150086 |
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| 基金项目： | 辽宁省博士启动基金资助项目 |
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| 摘 要： | 以45份具有不同程度抗旱性的大豆为材料,利用SSR分子标记对这些材料进行分析,选取2个产地、农艺性状差异显著的大豆品种静乐黑滚豆和辽13,对SSR引物进行筛选,从中选择出多态性丰富,带型较好的引物11对.用筛选出的引物对45份大豆种质进行PCR扩增.聚类结果表明:GD=0.41处可将45个大豆品种分成两大类,大部分品种被归为第1类,有31个品种,占总品种数的69%,第2类包含14品种,占总品种数的31%.两个大类又各自细分成不同的亚类群,聚类结果反映出品种间关系与地理起源、表型形态等具有一定的相关性.
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| 关 键 词： | 大豆 遗传多样性 SSR 聚类分析 |
SSR Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance |
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| WANG Zhen-dong,CHEN Chao-li,YU Bai-shuang,LI Jin-rong,WANG Hui.SSR Analysis of Genetic Diversity of Soybean Germplasm in Drought Resistance[J].Soybean Science,2010,29(3). |
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| Authors: | WANG Zhen-dong CHEN Chao-li YU Bai-shuang LI Jin-rong WANG Hui |
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| Institution: | WANG Zhen-dong1,CHEN Chao-li1,YU Bai-shuang2,LI Jin-rong2,WANG Hui1(1.Department of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,Liaoning,2.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China) |
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| Abstract: | The genetic diversity of 45 soybean germplasms including different degrees of drought resistance were analyzed by SSR method.Eleven pair of primers with rich polymorphism and good model were selected from'Jingle black'and'Liao 13',of which the place of origin and the agronomic traits are farther apart.The 11 pair of primers were used to amplify the genome DNA of 45 materials.Clustering results showed that 45 soybean varieties could be divided into two major categories while GD =0.41,most of the varieties we... |
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| Keywords: | SSR |
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