Inhibition of polygalacturonase and polygalacturonic acid lyase fromErwinia carotovora subsp.carotovora by phenolics in vitro |
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| Authors: | G D Lyon Fiona M McGill |
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| Institution: | (1) Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland, UK |
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| Abstract: | Summary The phenolic acids benzoic, caffeic, chlorogenic, ferulic, p-coumaric, protocatechuic, salicylic, sinapic, syringic and vanillic
together with vanillin, were tested for their ability to inhibit polygalacturonic acid lyase (PL) and polygalacturonase (PG)
in culture filtrates ofErwinia carotovora subsp.carotovora. None of the compounds inhibited PL at 200 μg/ml, although syringic and sinapic acids caused a 54% and 43% reduction respectively
at 400 μg/ml. PG was strongly inhibited by caffeic acid (57% inhibition at 100 μg/ml) and chlorogenic acid caused slight inhibition.
Little if any inhibition of PG was caused by benzoic, ferulic, p-coumaric, protocatechuic, salicylic, sinapic, syringic, and
vanillic acids or vanillin. The percentage inhibition of PG by chlorogenic acid was not affected by pH values over the range
4–7. |
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| Keywords: | tyrosinase caffeic acid chlorogenic acid ferulic acid pectic enzyme |
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