Identification for H. villosa chromatin in wheat lines using genomic in situ hybridization, C-banding and gliadin electrophoresis techniques |
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| Authors: | MQ Yu J Chen GB Deng M Cerbah XR Ma O Panaud S Yakovlev |
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| Institution: | (1) Chengdu Institute of Biology, Chinese Academy of Sciences, 610041 Chengdu, China;(2) Chengdu Institute of Biology, Chinese Academy of Sciences, 610041 Chengdu, China;(3) Laboratoire d'Evolution et Systematique, Universite Paris-Sud, 91405 ORSAY, France |
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| Abstract: | Detection of H. villosa chromosomes in telosomic addition and translocation lines of common wheat was undertaken using genomic in situ hybridization (GISH), C-banding techniques and polyacrylamide gels electrophoresis. The result of GISH on mitotic metaphase
cells of the addition line `95039' indicated that the added telochromosomes originated from H. villosa, and it was probably 6VS or 7Vs of H. villosa according to the C-banding pattern. Furthermore, the analysis of gliadin profiles demonstrated that the telochromosome was
6VS. A pair of 1RS/1BL translocation chromosome was also found in `95039'. In addition, mitotic GISH analysis showed that
the 6VS/6AL translocation chromosome remained unchanged after being transferred into new wheat background.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | C-banding gliadin genomic in situ hybridization (GISH) telosomic addition line translocation line |
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