| 不同方法提取苦荞蛋白的体外抗氧化活性研究 |
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| 引用本文： | 何晓兰,雷霆雯,许庆忠,王筑婷,莫晓川,李红梅.不同方法提取苦荞蛋白的体外抗氧化活性研究[J].安徽农业科学,2017,45(25). |
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| 作者姓名： | 何晓兰 雷霆雯 许庆忠 王筑婷 莫晓川 李红梅 |
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| 作者单位： | 贵州省人民医院病理科,贵州贵阳,550002;贵州医科大学基础医学院生物化学与分子生物学教研室,贵州贵阳,550025 |
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| 基金项目： | 贵州省科技计划项目，贵阳医学院博士科研启动基金 |
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| 摘 要： | 目的]研究不同方法提取苦荞蛋白的体外抗氧化活性。方法]以贵州威宁种植的苦荞为材料,通过蛋白质含量测定、清除羟基自由基能力测定对碱溶酸沉法提取苦荞粗蛋白和Osborne法分级提取苦荞清蛋白、球蛋白、醇溶蛋白、谷蛋白进行了比较分析。结果]通过碱溶酸沉法提取能获得大量的可溶性蛋白,Osborne法提取的蛋白虽然含量较低但获得的种类较多;各种蛋白均具有清除羟基自由基的能力,其清除羟基自由基能力从大到小依次为清蛋白、碱溶酸沉法提取的粗蛋白、谷蛋白、球蛋白、醇溶蛋白。结论]碱溶酸沉法和Osborne法提取的苦荞蛋白均具有体外抗氧化能力。
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| 关 键 词： | 苦荞蛋白 碱溶酸沉法 Osborne法 抗氧化活性 |
Study on Antioxidant Activity in Vitro of Tartary Buckwheat Proteins Extracted by Different Methods |
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| HE Xiao-lan,LEI Ting-wen,XU Qing-zhong,Wang Zhu-ting,Mo Xiao-chuan,LI Hong-mei.Study on Antioxidant Activity in Vitro of Tartary Buckwheat Proteins Extracted by Different Methods[J].Journal of Anhui Agricultural Sciences,2017,45(25). |
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| Authors: | HE Xiao-lan LEI Ting-wen XU Qing-zhong Wang Zhu-ting Mo Xiao-chuan LI Hong-mei |
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| Abstract: | Objective] The research aimed to study the antioxidant activity in vitro of tartary buckwheat proteins by different methods.Meth-od] Tartary buckwheat proteins was prepared by alkali-solution and acid-isolation, and albumin, globulin, prolamin and glutelin from tartary buckwheat were obtained by different solubility extraction.The coomassie brilliant blue protein quantitative was used in determination of pro-tein concent and chemical colorimetric method was used to determinate the ability to remove hydroxyl radicals( · OH) of each protein fraction. Result] The results showed that proteins obtained by alkali-solution and acid-isolation methos were the predominant fraction.Proteins extrac-ted by different methods had the ability to remove hydroxyl radicals, and the order of radical scavenging activity was albumin, proteins ob-tained by alkali-solution and acid-isolation methods, glutelin, globulin and prolamin. Conclusion] Both the alkali-soluble acid precipitation method and the Osborne method have the antioxidant capacity of tartary buckwheat protein. |
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| Keywords: | Tartary buckwheat proteins Alkali-solution and acid-isolation method Osborne method Antioxidant activity |
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