| 软枣猕猴桃"寒玉1号"组培技术研究 |
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| 引用本文： | 杜鹏瑶,刘德江,徐彪,赵佳明,申健.软枣猕猴桃"寒玉1号"组培技术研究[J].安徽农业科学,2017,45(22). |
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| 作者姓名： | 杜鹏瑶 刘德江 徐彪 赵佳明 申健 |
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| 作者单位： | 佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007;佳木斯大学生命科学学院,黑龙江佳木斯,154007 |
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| 基金项目： | 佳木斯大学应用技术类项目,佳木斯大学大学生创新项目 |
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| 摘 要： | 目的]探索软枣猕猴桃"寒玉1号"适宜组培方法。方法]以新生腋芽为外植体,筛选最佳消毒方法,并对影响其生长的NAA、6-BA等生长调节物质的浓度进行筛选。结果]以5%NaClO 8 min+0.1%HgCl_26 min或9 min,5%NaClO 10 min+0.1%HgCl_26 min为适宜消毒方法,污染率和褐化率均较低;腋芽在WPM+6-BA 3.0 mg/L+NAA 0.2 mg/L培养基中增殖效果最佳,增殖系数可达5.6,株高3.9 cm;丛生芽在WPM+NAA 0.3 mg/L培养基中诱导生根效果最佳,生根率可达100%,平均根条数为4.4条,平均根长为5.2 cm。结论]软枣猕猴桃"寒玉1号"可采用组织培养的方法进行快速繁殖。
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| 关 键 词： | 软枣猕猴桃 "寒玉1号" 腋芽 组织培养 |
Tissue Culture Technique for Actinidia arguta "Hanyu No.1" |
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| DU Peng-yao,LIU De-jiang,XU Biao,Zhao Jia-ming,SHEN Jian.Tissue Culture Technique for Actinidia arguta "Hanyu No.1"[J].Journal of Anhui Agricultural Sciences,2017,45(22). |
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| Authors: | DU Peng-yao LIU De-jiang XU Biao Zhao Jia-ming SHEN Jian |
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| Abstract: | Objective] To explore the suitable tissue culture technique for Actinidia arguta "Hanyu No.1".Method] With axillary bud as explant,the best disinfection method was filtered,and the NAA,6-BA concentration were selected.Result] The best disinfection methods were 5% NaClO 8 min+0.1%HgCl2 6 min or 9 min,5% NaClO 10 min+0.1%HgCl2 6 min,in which the mortality rate and contamination rate were lower.The best medium of inducing germinating of explant was WPM+6-BA 3.0 mg/L +NAA 0.2 mg/L,in which the ratio of proliferation was 5.6 and the height of plant was 3.9 cm.The best medium of inducing rooting from cluster buds was WPM+NAA 0.3 mg/L,in which the rate of rooting was 100%,the average number of root was 4.4 and the average length of root was 5.2 cm.Conclusion] It concluded that Actinidia arguta "Hanyu No.1" can adopt the method of tissue culture for rapid propagation. |
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| Keywords: | Actinidia arguta "Hanyu No 1" Axillary bud Tissue culture |
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