| Folin-Ciocalteu比色法测定头花蓼多酚含量 |
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| 引用本文： | 江楠,李潇彬,顾曼琦,廖莉玲.Folin-Ciocalteu比色法测定头花蓼多酚含量[J].安徽农业科学,2017,45(23). |
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| 作者姓名： | 江楠 李潇彬 顾曼琦 廖莉玲 |
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| 作者单位： | 贵州轻工职业技术学院,贵州贵阳,550025;贵州师范大学化学与材料科学学院,贵州贵阳,550001 |
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| 基金项目： | 贵州省科技厅_2014年度贵州省科技厅与贵州师范大学联合基金计划项目 |
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| 摘 要： | 目的]建立头花蓼中多酚含量的比色测定方法。方法]以没食子酸为对照品,对测定头花蓼中多酚含量的比色条件(包括检测波长、Na_2CO_3用量、Folin-Ciocalteu试剂用量、反应温度及时间对吸光值的影响)进行了研究。结果]当FC试剂用量为2.5 mL,12%Na_2CO_3用量为7 mL时,在35℃下反应40 min为最佳反应条件;多酚的含量与760 nm处吸光度呈良好的线性关系;测定方法的平均回收率为98.66%,RSD为0.380%。结论]该方法不仅操作简单,而且稳定性、重现性、精密度好,能够用于头花蓼提取液多酚含量的测定。
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| 关 键 词： | 头花蓼 多酚 Folin-Ciocaheu比色法 检测条件 |
Determination of Polyphenols in Polygonum capitatum by Folin-Ciocalteu Colorimetry |
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| JIANG Nan,LI Xiao-bin,GU Man-qi,LIAO Li-ling.Determination of Polyphenols in Polygonum capitatum by Folin-Ciocalteu Colorimetry[J].Journal of Anhui Agricultural Sciences,2017,45(23). |
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| Authors: | JIANG Nan LI Xiao-bin GU Man-qi LIAO Li-ling |
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| Abstract: | Objective] The research aimed to establish a colorimetric method for the determination of polyphenols in Polygonum capitatum.Method]To gallic acid as a reference substance,the colorimetric conditions (the effects of detection wavelength,Na2CO3 dosage,Folin-Ciocalteu reagent dosage,reaction temperature and time on the absorbance value) were studied.Result] When Folin-Ciocalteu reagent was 2.5 mL and 12％ sodium carbonate solution was 7 mL,reaction 40 min at 35 ℃,there was a good linearity between the absorbance and gallic acid concentration in 760 nm,the average recovery was 98.66％ and RSD was 0.380％.Conclusion] This method is simple,accurate,repeatable and stable.It can be used for the determination of polyphenol content in Polygonum capitatum. |
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| Keywords: | Polygonum capitatum Polyphenol Folin-Ciocaileu colorimetry Detecting condition |
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