| 福建省梨地方品种的遗传多样性研究 |
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| 引用本文： | 岳晓燕,黄新忠,宗宇,滕元文.福建省梨地方品种的遗传多样性研究[J].园艺学报,2015,42(1):119-123. |
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| 作者姓名： | 岳晓燕 黄新忠 宗宇 滕元文 |
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| 作者单位： | 1浙江大学园艺系，农业部园艺植物生长发育与品质调控重点开放实验室，杭州 310058；2福建省农业科学院果树研究所，福州 350013 |
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| 基金项目： | 高等学校博士学科点专项科研基金项目（20110101110091） |
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| 摘 要： | 利用叶绿体DNA非编码区序列acc D-psa I和17对SSR引物对原产于福建省的49个梨地方品种的遗传多样性进行分析,旨在为育种利用提供参考。获得49份福建地方品种的acc D-psa I序列,并检测到4个多态性位点,包含5个叶绿体单倍型(Hap1~Hap5),其中核苷酸多态性(Pi)为0.00139,单倍型多样性(Hd)为0.660。TCS网络图显示Hap5是最古老的单倍型,其仅在两个样品中检测到。17个SSR位点共检测到211个等位基因位点,有效等位基因数(A)为5~23个,平均值为12.41;观察杂合度(Ho)和期望杂合度(He)的平均值分别为0.5802和0.7549;17个SSR位点的Shannon’s信息指数(I)值为0.5830~2.7683,平均值为1.8661,表现出较高的遗传多样性。基于Nei和Li的遗传距离的邻接法(Neighbor-Joining,NJ)将49份样品聚为9个组,其中大部分聚类结果与单倍型类型表现的亲缘关系较为一致。叶绿体单倍型和SSR多态性位点信息证实福建地方品种具有较为丰富的遗传多样性。
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| 关 键 词： | 梨 地方品种 SSR cpDNA 遗传多样性 亲缘关系 |
Genetic Diversity of Cultivated Pears from Fujian Province Revealed by cpDNA Haplotypes and Nuclear Microsatellites |
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| YUE Xiao-yan,HUANG Xin-zhong,ZONG Yu,TENG Yuan-wen.Genetic Diversity of Cultivated Pears from Fujian Province Revealed by cpDNA Haplotypes and Nuclear Microsatellites[J].Acta Horticulturae Sinica,2015,42(1):119-123. |
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| Authors: | YUE Xiao-yan HUANG Xin-zhong ZONG Yu TENG Yuan-wen |
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| Institution: | 1. Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China;
2. Department of Cardiothoracic Surgery, Nanhai People's Hospital, Foshan 528200, China;
3. Department of Hepatobi-liary Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China |
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| Abstract: | A non-coding chloroplast region，accD-psaI and 17 microsatellite markers were used to assay genetic diversity of 49 pear accessions from Fujian Province. A total of 49 sequences were collected and five haplotypes（Hap1–Hap5）were recognized containing four variation sites. Nucleotide diversity（Pi）and haplotype diversity were 0.00139 and 0.660. The TCS Network indicated that Hap5 was the ancestral haplotype，only detected in two individuals. Two hundred and eleven different alleles were detected among seventeen SSR loci，ranged from 5 to 23 with an average of 12.41 alleles per locus. The mean observed and expected heterozygosity values were 0.5802 and 0.7549，respectively. The Shannon’s information index for each locus was from 0.5830 to 2.7683 with a mean value of 1.8661. The Neighbor-Joining（NJ）tree grouped 49 pear accessions to nine major groups，which are generally congruent to haplotype diversity. Chloroplast haplotypes and microsatellite locus polymorphism information demonstrated a high level of genetic diversity of pear landraces from Fujian，China. |
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| Keywords: | pear landraces SSR cpDNA genetic diversity genetic relationship |
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