Effects of rested-harvest using the anesthetic AQUI-S? on channel catfish,Ictalurus punctatus,physiology and fillet quality |
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| Institution: | 1. Animal Welfare Laboratory, Agricultural Sciences Sector, Federal University of Paraná, Brazil. Rua dos Funcionários 1540, Juvevê, Curitiba, Paraná CEP: 80035-050, Brazil;2. Research Station in Fish Farming and Ecology of Native Species of ITAIPU Binacional, Foz do Iguaçu, Paraná CEP: 85856-970, Brazil;1. College of Fisheries, Hubei Provincial Engineering Laboratory for Pond Aquaculture, National Demonstration Center for Experimental Aquaculture Education, Huazhong Agricultural University, Wuhan 430070, PR China;2. Department of Animal Production, Faculty of Agriculture, Mansoura University, Al-Mansoura 35516, Egypt |
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| Abstract: | Trials were conducted to determine effects of AQUI-S? sedation during harvest (rested-harvest) on physiological responses and fillet quality of channel catfish, Ictalurus punctatus. Rested-harvest is defined as application of an anesthetic immediately before harvest to reduce fish activity associated with a normal harvest. Doses of 25–35 ppm AQUI-S? were effective for rested-harvest of catfish (loss of equilibrium in 3 to 10 min and 100% survival following recovery). Time to loss of equilibrium and time to recovery following sedation with 35 ppm AQUI-S? increased as water temperature decreased from 30 °C to 10 °C. Catfish exposed to 25 ppm AQUI-S?, 35 ppm AQUI-S?, 100 ppm trincaine methanesulfonate, and 8 ppm metomidate had lower blood lactate, cortisol, and glucose and higher blood pH than unsedated fish exposed to a low-water stress. Rested-harvest (RH) catfish had higher muscle and blood pH, lower blood and muscle lactate, and higher muscle ATP levels than catfish exposed to a 45 min low-water stress. Rates of muscle pH decrease, ATP decrease, and lactate increase accelerated as storage temperature decreased from 15 °C to 5 °C in RH fish acclimated to summer temperatures (33 °C), conversely these rates accelerated as storage temperature increased from 5 °C to 15 °C in RH fish acclimated to winter temperatures (7 °C). Based on physiological response (higher muscle pH, lower blood lactate, delayed time to rigor), post-sedation euthanasia by CO2 was superior to post-sedation euthanasia by AQUI-S? overdose (150 ppm), nitrogen gas, or electrical stunning. Compared to fillets from fish exposed to simulated industry transport conditions, fillets from RH/CO2 euthanised fish had higher pH 1 h post-slaughter, and less drip-loss and lower L? and a? color values during 7 days of iced storage. RH/CO2 and control fillets were not different for shelf-life based on bacterial counts. Rested-harvest with AQUI-S? followed by CO2 euthanasia has potential to improve catfish fillet quality, but AQUI-S? approval, development of rested-harvest strategies, and demonstration of economic benefits of rested-harvest will be required for adoption of rested-harvest to commercial catfish production. |
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