| 鹅源高活力纤维素分解菌的分离鉴定 |
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| 引用本文： | 张名爱,王宝维.鹅源高活力纤维素分解菌的分离鉴定[J].中国家禽,2012,34(5):12-15. |
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| 作者姓名： | 张名爱 王宝维 |
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| 作者单位： | 青岛农业大学食品科学与工程学院,山东青岛,266109 |
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| 基金项目： | 国家水禽产业技术体系(CARS-43-11);山东省农业良种产业化项目(2010LZ015-04-02) |
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| 摘 要： | 利用纤维素刚果红培养基初筛,滤纸失重和酶活测定试验复筛,从鹅盲肠筛选到1株纤维素分解能力较强的真菌F67。经中国科学院微生物研究所鉴定,F67为草酸青霉,微生物保藏号为CGMCC NO.2260。该菌在以纤维素为唯一碳源的培养液中,28℃振荡培养8 d,滤纸纤维素失重率达28.53%,滤纸酶活力(FPA)可达449.79 U/g。试验结果还表明,真菌分解纤维素的能力最强,放线菌次之,细菌最差。
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| 关 键 词： | 纤维素分解菌 鹅 盲肠 分离 |
Isolation and Identification of a High Active Cellulose-decomposing Strain from Geese |
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| ZHANG Ming'ai
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WANG Baowei.Isolation and Identification of a High Active Cellulose-decomposing Strain from Geese[J].China Poultry,2012,34(5):12-15. |
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| Authors: | ZHANG Ming'ai WANG Baowei |
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| Institution: | (College of Food Science and Engineering, Qingdao Agricultural University,Qingdao,Shandong 266109) |
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| Abstract: | Using cellulose-congo red medium for the first screening and in combination of the filter paper weight losing test and enzyme activity measuring test,a high cellulose-decomposing bacteria named F67 was obtained from the caecum of geese.It was cultured in a liquid medium with cellulose as the sole carbon source.Culturing at 28 ℃ for 8 days,the decomposed ratio of the filter paper cellulose was 28.53% and the filter paper enzyme activity(FPA) was 449.79 U/g.Through authenticating,F67 was Penicillium oxalicum Currie & Thom.The results showed that epiphyte had the best ability to decompose crude fiber,the following was actinomyces and the worst was bacterium. |
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| Keywords: | Cellulose-decomposing bacteria goose caecum isolation |
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